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DNA Reagents

Chemical reagents and other products used for DNA-related techniques and processes such as PCR cloning, DNA sequencing, DNA cloning and mapping, mutagenesis, DNA synthesis, etc.; includes kits, columns, buffers, labelling technology, and others.
DNA Sequencing (307)
PCR Cloning Kits (100)
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1 – 15 of 378 results
1
Promotions Available

Invitrogen™ TOPO™ TA Cloning™ Kit for Subcloning, without competent cells

EDGE
Antibiotic Resistance Bacterial Ampicillin (AmpR), Kanamycin (KanR)
Format Kit
Product Type Cloning Kit
Cloning Method TOPO™-TA
Product Line TOPO™
2

Thermo Scientific™ CloneJET PCR Cloning Kit

Efficiently clone PCR products generated with any thermostable DNA polymerase using Thermo Scientific™ CloneJET™ PCR Cloning Kit, a positive selection system.

EDGE
Antibiotic Resistance Bacterial Ampicillin (AmpR)
Format Kit
Sample Type PCR Products
Cloning Method Restriction Enzyme⁄ MCS, Blunt PCR
For Use With (Application) PCR Cloning
Vector pJET1.2/blunt
Product Line CloneJET
3

Thermo Scientific™ Rapid DNA Ligation Kit

Enable fast sticky-end or blunt-end DNA ligation in only 5 minutes at room temperature.

EDGE
Product Type Rapid DNA Ligation Kit
Enzyme DNA Ligase
4
Promotions Available

Thermo Scientific™ pUC18 DNA

Optimize cloning with this double-stranded closed circular high copy plasmid DNA, 2686 base pairs with a molecular weight of 1.74 x 106 Da isolated from E.coli.

EDGE
5

Applied Biosystems™ BigDye™ Direct Cycle Sequencing Kit

The BigDye™ Direct Cycle Sequencing Kit, part of the BigDye™ product family, is a complete kit for your PCR and Sequencing needs.

EDGE
Shipping Condition Dry Ice
Format 8-well Strip,96-well Plate
Product Type Direct Cycle Sequencing Kit
Technique Fluorescent Dye Terminator Sequencing
For Use With (Application) Comparative Sequencing (Germline Mutations - 30:70 Heterozygotes), Comparative Sequencing (Germline Mutations - 50:50), GC-Rich Sequencing (>65%), GT-Rich or Difficult Template Sequencing, Standard Sequencing, Gap Closure, Homopolymer A or T, AT-Rich Sequencing (>65%), Comparative Sequencing (Germline Mutations -10:90 Heterozygotes)
Template Compatibility BAC DNA, Cosmids, Lambda DNA, PCR Amplicons (10:90 Heterozygotes), Plasmid DNA (≤15Kb), Genomic DNA (Bacterial), Fosmids, PCR Amplicons (50:50 Heterozygotes), PCR Amplicons (30:70 Heterozygotes)
Product Line BigDye™
6
Promotions Available

Invitrogen™ Zero Blunt™ TOPO™ PCR Cloning Kit, with One Shot™ TOP10 Chemically Competent E. coli cells

Provides a highly efficient, 5-minute, one-step cloning strategy (TOPO Cloning) for the direct insertion of blunt-ended PCR products amplified with proofreading thermostable polymerases into a plasmid vector

EDGE
Promoter SP6
Content And Storage Box 1:
• Linearized and topoisomerase I-activated pCR™Blunt II-TOPO™ vector
• Salt solution
• dNTPs
• Control template
• M13 forward and reverse primers
• Sterile water

Store at -5 to -30°C.
All reagents are stable for 6 months when properly stored.

Box 2:
• One Shot™ Chemically Competent or Electrocomp™ E. coli
• S.O.C. medium
• Supercoiled pUC19 control plasmid

Store at -68 to -85°C.
Format Kit
Product Type PCR Cloning Kit
Cloning Method Blunt TOPO™
Bacterial or Yeast Strain TOP10
Cell Type Chemically Competent
7
Promotions Available

Applied Biosystems™ BigDye™ Terminator v3.1 Cycle Sequencing Kit

The BigDye™ Terminator v3.1 Cycle Sequencing Kit's robust, highly flexible chemistry is ideal for de novo sequencing, resequencing, and finishing with PCR product, plasmid, fosmid, and BAC templates.

Format 12-well Strips,8-well Strip,96- or 384-well Plate
Product Type Sequencing Buffer
For Use With (Equipment) SeqStudio™ Genetic Analyzer, SeqStudio™ Flex Series Genetic Analyzer, 3500/3500xL Genetic Analyzer, 3730/3730xl DNA Analyzer Thermal Cyclers: GeneAmp™ PCR System 9700 Dual Series, Veriti™ Fast 96‐well and 384‐well Thermal Cyclers
Technique Fluorescent Dye Terminator Sequencing
Product Line BigDye™ Terminator
8

Invitrogen™ Zero Blunt™ TOPO™ PCR Cloning Kit, without competent cells

Promoter SP6
Content And Storage Box 1:
• Linearized and topoisomerase I-activated pCR™Blunt II-TOPO™ vector
• Salt solution
• dNTPs
• Control template
• M13 forward and reverse primers
• Sterile water

Store at -5 to -30°C.
All reagents are stable for 6 months when properly stored.
Antibiotic Resistance Bacterial Kanamycin (KanR), Zeocin™ (ZeoR)
Format Kit
Product Type PCR Cloning Kit
Cloning Method Blunt TOPO™
9
Promotions Available

Applied Biosystems™ 3500xL Genetic Analyzer 24-Capillary Array, 36 cm

Use for fragment analysis and rapid sequencing applications.

10
Promotions Available

Applied Biosystems™ Hi-Di™ Formamide

Highly deionized (Hi-Di) formamide

11

Applied Biosystems™ 3730 Running Buffer (10X)
GREENER_CHOICE

The concentrated Running Buffer is used for electrophoresis on Applied Biosystems™3730 and 3730XL DNA Analyzers.

12

Invitrogen™ GeneArt™ Gibson Assembly HiFi Cloning Kit, electrocompetent cells

The GeneArt Gibson Assembly HiFi Cloning Kit enables DNA assembly and cloning via a technique that allows multiple overlapping DNA fragments to be seamlessly linked in a one-step, 15–60 minute isothermal reaction.

13
Promotions Available

Invitrogen™ GeneArt™ Gibson Assembly HiFi Master Mix

GeneArt Gibson Assembly HiFi Master Mix enables DNA assembly and cloning via a technique that allows multiple overlapping DNA fragments to be seamlessly linked in a one-step, 15–60 minute isothermal reaction.

EDGE
Shipping Condition Ambient, Wet Ice, Dry Ice
Content And Storage Includes master mix, positive control, and water and accommodates the use of your own competent cells.
Workflow Step DNA Assembly
Number of Fragments Up to 6 fragments
Product Type HiFi Master Mix
Control Type Positive Control
Cloning Method Seamless cloning
For Use With (Application) Seamless Cloning & Genetic Assembly
Product Line GENEART™
14

Applied Biosystems™ GeneScan™ 1200 LIZ™ dye Size Standard

The GeneScan™ 1200 LIZ™ Size Standard is a fifth dye (LIZ™) labeled high density size standard for the reproducible sizing of fragment analysis samples.

EDGE
15

Invitrogen™ GeneArt™ Gibson Assembly EX Master Mix

GeneArt Gibson Assembly EX Master Mix enables DNA assembly via a technique that allows multiple overlapping DNA fragments to be seamlessly linked in a single-tube, two-step optimized reaction.

EDGE
Shipping Condition Dry Ice
Content And Storage Includes master mix, positive control, and water and accommodates the use of your own competent cells.
Workflow Step DNA Assembly
Number of Fragments Up to 15 fragments
Product Type Master Mix
Control Type Positive Control
Cloning Method Seamless cloning
For Use With (Application) Seamless Cloning & Genetic Assembly
Product Line GENEART™