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DNA Sequencing

Chemical reagents, kits, and other products used for DNA sequencing; includes sequencing and pre-sequencing kits, purification kits, buffers, standards, etc.
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Invitrogen™ Collibri™ Library Quantification Kit

Invitrogen™ Collibri™ Library Quantification Kit includes a ready-to-use master mix optimized for Illumina NGS library quantification and a library dilution buffer

EDGE
Shipping Condition Dry Ice
Polymerase Platinum™ II Taq Hot Start DNA Polymerase
GC-Rich PCR Performance Low
Content And Storage Store at -20°C.
Format Kit
Product Type Library Quantification Kit
For Use With (Equipment) 7500 Fast System, 7900HT Fast System, QuantStudio™, ViiA™ 7 System, StepOne™ System, StepOnePlus™ System
Sample Type DNA,RNA
PCR Method qPCR
For Use With (Application) Illumina NGS Library Quantification
Test Time Standard
Product Line Collibri
Detection Method SYBR
3

Applied Biosystems™ BigDye™ Terminator v1.1 & v3.1 5X Sequencing Buffer

This sequencing buffer is optimized for use with BigDye™ Terminator v1.1 and v3.1 Cycle Sequencing Kits.

EDGE
Shipping Condition Room Temperature
Buffer Sequencing Buffer
Content And Storage Store at 2°C to 8°C.
Form Liquid
Concentration 5X
For Use With (Application) Standard Sequencing
4

Applied Biosystems™ BigDye™ Terminator v1.1 Cycle Sequencing Kit

This BigDye™ Terminator v1.1 Cycle Sequencing Kit is designed for specialty applications that require optimal basecalling adjacent to the primer and for sequencing short PCR product templates with rapid electrophoresis run modules.

Product Type Sequencing Buffer
For Use With (Equipment) SeqStudio™ Genetic Analyzer, SeqStudio™ Flex Series Genetic Analyzer, 3500/3500xL Genetic Analyzer, 3730/3730xl DNA Analyzer Thermal Cyclers: GeneAmp™ PCR System 9700 Dual Series, Veriti™ Fast 96‐Well and 384‐Well Thermal Cyclers
Technique Fluorescent Dye Terminator Sequencing
Template Compatibility BAC DNA, Plasmid DNA (≤15 Kb), PCR Amplicons, Single Stranded DNA
Product Line BigDye™ Terminator
5

Applied Biosystems™ BigDye™ Terminator v3.1 Cycle Sequencing Kit

The BigDye™ Terminator v3.1 Cycle Sequencing Kit's robust, highly flexible chemistry is ideal for de novo sequencing, resequencing, and finishing with PCR product, plasmid, fosmid, and BAC templates.

Format 12-well Strips,8-well Strip,96- or 384-well Plate
Product Type Sequencing Buffer
For Use With (Equipment) SeqStudio™ Genetic Analyzer, SeqStudio™ Flex Series Genetic Analyzer, 3500/3500xL Genetic Analyzer, 3730/3730xl DNA Analyzer Thermal Cyclers: GeneAmp™ PCR System 9700 Dual Series, Veriti™ Fast 96‐well and 384‐well Thermal Cyclers
Technique Fluorescent Dye Terminator Sequencing
Product Line BigDye™ Terminator
6

Applied Biosystems™ BigDye™ Direct Cycle Sequencing Kit

The BigDye™ Direct Cycle Sequencing Kit, part of the BigDye™ product family, is a complete kit for your PCR and Sequencing needs.

EDGE
Shipping Condition Dry Ice
Format 8-well Strip,96-well Plate
Product Type Direct Cycle Sequencing Kit
Technique Fluorescent Dye Terminator Sequencing
For Use With (Application) Comparative Sequencing (Germline Mutations - 30:70 Heterozygotes), Comparative Sequencing (Germline Mutations - 50:50), GC-Rich Sequencing (>65%), GT-Rich or Difficult Template Sequencing, Standard Sequencing, Gap Closure, Homopolymer A or T, AT-Rich Sequencing (>65%), Comparative Sequencing (Germline Mutations -10:90 Heterozygotes)
Template Compatibility BAC DNA, Cosmids, Lambda DNA, PCR Amplicons (10:90 Heterozygotes), Plasmid DNA (≤15Kb), Genomic DNA (Bacterial), Fosmids, PCR Amplicons (50:50 Heterozygotes), PCR Amplicons (30:70 Heterozygotes)
Product Line BigDye
7

Invitrogen™ Collibri™ PCR-free ES DNA Library Prep Kits for Illumina Systems

The Invitrogen Collibri PCR-free ES DNA Library Preparation kits are designed for next-generation sequencing (NGS) on Illumina systems. The kit supports whole-genome sequencing (WGS) applications and is suitable for all genomes.

Format Kit
Workflow Step Library Generation
Libraries Fragment Library
For Use With (Application) Whole Genome Sequencing, Resequencing, De Novo Assembly
Sequencing Type Genome and DNA Sequencing
Product Line Collibri
8

Applied Biosystems™ BDT/POP/Buffer Bundle

The BDT/POP/Buffer Bundle includes a BigDye Terminator 3.1 Cycle Sequencing Kit, POP-7 Polymer, Anode Buffer Containers, and Cathode Buffer Containers for your 3500 or SeqStudio Flex genetic analyzer. All products are offered in a single, easy-to-order bundle to provide key reagents for Sanger sequencing workflows.

Format Kit
Product Type Sequencing Buffer
Enzyme Proprietary
Control Type Plasmid DNA
Sample Type Purified DNA
Isolation Technology Enzymatic, Magnetic Bead
Label or Dye Various
For Use With (Application) Standard Sequencing
Species Various
Sequencing Type Genome and DNA Sequencing
Detection Method Fluorescent Dye
9

Invitrogen™ Collibri™ PCR-free PS DNA Library Prep Kits for Illumina Systems

The Invitrogen Collibri PCR-free PS DNA Library Preparation kits are designed for next-generation sequencing (NGS) on Illumina systems. The kit supports whole-genome sequencing (WGS) applications and is suitable for all genomes, including FFPE samples.

Shipping Condition Dry Ice
Format Kit
Workflow Step Library Generation
Sample Type DNA
Libraries Fragment Library
For Use With (Application) Whole Genome Sequencing, Resequencing, De Novo Assembly
Sequencing Type Genome and DNA Sequencing
Product Line Collibri
10

Invitrogen™ Collibri™ ES DNA Library Prep Kits for Illumina Systems

The Invitrogen Collibri ES DNA Library Preparation kits are designed for next-generation sequencing (NGS) on Illumina systems. The kit supports whole-genome sequencing (WGS) applications and is suitable for all genomes.

Workflow Step Library Generation
Libraries Fragment Library
For Use With (Application) Whole Genome Sequencing, Resequencing, De Novo Assembly
Sequencing Type Genome and DNA Sequencing
Product Line Collibri
11

Applied Biosystems™ MicroSEQ™ ID Microbial Identification Software v4.0, with all libraries

MicroSEQ ID Microbial Identification Software, version 4.0, allows users to easily identify and classify bacterial or fungal sequences by comparing them to a validated microbial library.

12

Applied Biosystems™ POP-7™ Polymer For 3500 Dx Series

Conveniently offered in easy to use pouch packages

Product Type POP-7™ Polymer
For Use With (Equipment) 3500 Dx Series
Size 1 Kit
13

Applied Biosystems™ Sequenase Version 2.0 DNA Polymerase

Sequenase™ Version 2.0 DNA Polymerase is a genetically engineered form of T7 DNA polymerase. Unlike the wild-type enzyme it has virtually no 3'→5' exonuclease activity.

EDGE
Polymerase Genetically-Engineered form of T7 DNA Polymerase
Product Type Sequenase Version 2.0 DNA Polymerase
Purity 0.95
Concentration 13 units/μL
For Use With (Application) Sequencing
14

Applied Biosystems™ Sanger Sequencing Kit

Sanger Sequencing Kit includes all reagents needed for sample preparation for sanger sequencing

15

Invitrogen™ Collibri™ DNA Library Preparation Kit Modules

The Invitrogen Collibri DNA Library Preparation Kit Modules are designed for a custom build of next-generation sequencing (NGS) workflow on Illumina systems.

Format Kit
For Use With (Application) DNA Sequencing; Whole-Genome Sequencing, Amplicon Sequencing
Sequencing Type Genome and DNA Sequencing
Product Line Collibri