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Gene Reporter Systems

Reporter genes are attached to a regulatory sequence of a gene of interest. When expressed by the host organism, they generate measurable, easily identifiable characteristics that can be used as an indication of whether a gene has been taken up or expressed.
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1
Promotions Available

Invitrogen™ Biotin-14-dCTP

Used for DNA biotinylation, an excellent, and sometimes superior alternative to conventional radioisotopic labeling

2
Promotions Available

Invitrogen™ Yeast tRNA

Supplied in lyophilized form

EDGE
Shipping Condition Approved for shipment at Room Temperature or on Wet or Dry Ice
Content And Storage Store in freezer (-5 to -30°C).
Form Lyophilized Powder
Product Type Yeast tRNA
Reagent Type Yeast tRNA
For Use With (Application) Carrier for Nucleic Acid Precipitation
3

Invitrogen™ Phospha-Light™ SEAP Reporter Gene Assay System

Chemiluminescent reporter gene assay for sensitive detection of secreted human placental alkaline phosphatase used as a reporter protein.

EDGE
Content And Storage This kit contains the substrate, required buffers, enhancer, and control Placental Alkaline Phosphatase.
Store the product at 4°C.
Form Liquid
Readout End Point
Compatible Cells Mammalian Cells
Target Enzyme Alkaline Phosphatase
Substrate Type AP (Alkaline Phosphatase) Substrate
For Use With (Application) Enzyme Assay
High-throughput Compatibility High-throughput Compatible
Detection Method Chemiluminescence
Substrate CSPD™
Assay Reporter Assay
Substrate Properties Chemical Substrate
For Use With (Equipment) Luminometer (Microplate)
Product Type Secreted Human Placental Alkaline Phosphatase Reporter Gene Assay System
Sample Type Cell Cultures
Label or Dye Unconjugated
Product Line NovaBright, Phospha-Light
4

Invitrogen™ TOPO™ TA Cloning™ Kit for Sequencing, with One Shot™ TOP10 Chemically Competent E. coli

Provides a highly efficient, 5-minute, one-step cloning strategy (TOPO Cloning) for the direct insertion of Taq polymerase–amplified PCR products into a plasmid vector for sequencing

EDGE
Format Kit
Product Type Cloning Kit
Cloning Method TOPO™-TA
Bacterial or Yeast Strain TOP10
Vector TOPO-TA Cloning Vectors
Product Line One Shot
5

Invitrogen™ TC-FlAsH™ II In-Cell Tetracysteine Tag Detection Kit (Green Fluorescence), for live-cell imaging

Contains an expression tag-based fluorescence labeling reagent for live-cell labeling

EDGE
6

Invitrogen™ Human Cot-1 DNA™

Effective library-screening probe for somatic-cell hybrid libraries and flow-sorted chromosome libraries made from somatic-cell hybrids

7

Invitrogen™ Luc-Screen™ Extended-Glow Luciferase Reporter Gene Assay System

Thermo Scientific Luc-Screen Extended-Glow Luciferase Reporter Gene Assay System with extended-glow light emission is designed for sensitive detection of firefly luciferase reporter enzyme in microplate cell cultures. A linear signal is obtained with the

Form Liquid
Readout End Point
Compatible Cells Mammalian Cells
Target Enzyme Luciferase
Substrate Type Luciferase Substrate
For Use With (Application) Enzyme Assay
High-throughput Compatibility High-throughput Compatible
Detection Method Chemiluminescence
Substrate Luciferin
Assay Luciferase Assay
Substrate Properties Chemical Substrate
For Use With (Equipment) Luminometer (Microplate)
Product Type Luciferase Reporter Gene Assay System
Sample Type Cell Cultures
Product Line Luc-Screen, NovaBright
8

Invitrogen™ GLOBINclear™-Human Kit, for globin mRNA depletion

A rapid and robust treatment for RNA derived from whole blood that uses a novel, non-enzymatic technology to rapidly deplete >95% of the alpha and beta globin mRNA from total RNA preparations.

10

Invitrogen™ Alexa Fluor™ 647-aha-dCTP

For better resolution of two-color microarray gene expression assays

11

Invitrogen™ Human Cot-1 DNA™-Fluorometric QC

Quantifying by fluorometry provides greater data accuracy

12

Invitrogen™ NovaBright™ Phospha-Light™ EXP Assay Kit for SEAP (Secreted Placental Alkaline Phosphatase) Reporter Gene Detection

Chemiluminescent reporter gene assay for the ultrasensitive detection of secreted placental alkaline phosphatase or the non-secreted placental isozyme PLAP.

Substrate CSPD™
Substrate Properties Chemical Substrate
Content And Storage Component solution containing CSPD™ substrate and chemiluminescence enhancer (Store at 2°C to 6°C), placental alkaline phosphatase positive control (Store at ≤ -20°C)
Format 96-well Plate
For Use With (Equipment) Luminometer (Microplate)
Product Type Phosphatase Assay
Target Enzyme Placental Alkaline Phosphatase
Substrate Type AP (Alkaline Phosphatase) Substrate
High-throughput Compatibility High-throughput Compatible
Product Line NovaBright
Detection Method Enhanced Chemiluminescence
13

Invitrogen™ ImaGene Red™ C12RG lacZ Gene Expression Kit

Substrates cleaved within cell for fluorescent product

14

Invitrogen™ Galacto-Light Plus™ β-Galactosidase Reporter Gene Assay System

Rapid, ultrasensitive detection of β-galactosidase in reporter gene assays. Thermo Scientific Galacto-Light Plus β-Galactosidase Reporter Gene Assay System is designed for luminometers without injectors.

Content And Storage This kit contains the lysis solution, substrate, reaction buffer, and accelerator.
Store at 4°C.
Form Liquid
Readout End Point
Compatible Cells Mammalian Cells
Target Enzyme Beta-Galactosidase
Substrate Type Beta-Gal Substrate
For Use With (Application) Enzyme Assay
High-throughput Compatibility High-throughput Compatible
Detection Method Chemiluminescence
Substrate Galacton-Plus™
Assay β-Gal (lacZ) Assay
Substrate Properties Chemical Substrate
For Use With (Equipment) Luminometer (Microplate)
Product Type β-Galactosidase Reporter Gene Assay System
Sample Type Cell Cultures
Label or Dye Unconjugated
15

Invitrogen™ Random Primers DNA Labeling System

Ideal for radioactively labeling DNA, particularly fragments under 1 kb