Restriction Enzymes

Restriction enzymes cleave DNA into fragments at or near specific recognition sequences known as restriction sites; also known as restriction endonuclease, or restrictase. Products include various restriction enzymes, kits, buffers, etc.

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Thermo Scientific™ TheraPure™ Restriction Enzymes

Thermo Scientific TheraPure in vitro transcription (IVT) enzymes are designed for advanced research and early development of mRNA therapeutics and vaccines.

Pricing and Availability
Specifications

Cut Site	CTCTTC(1/4)^
Content And Storage	Store at -20°C.
Form	Liquid
Enzyme	Eam1104I
Methylation Sensitivity	Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive,Not CpG Methylation-Sensitive
Quantity	3000 U, 150 μL
Type IIS RE	Yes
Incubator Temperature	37°C
Concentration	20 U/μL
For Use With (Application)	In vitro Transcription
Inactivation Temperature	65°C
Sensitive to Heat Inactivation	Yes
Product Type	Restriction Enzyme
Research Category	Drug Development
Compatible Buffer	10X Tango Buffer
Optimal Reaction Temperature	37°C
Product Line	TheraPure

Thermo Scientific™ Buffer Set for Restriction Enzymes

Ensure the optimum reaction conditions for each restriction enzyme with this five buffer system.

Pricing and Availability

Thermo Scientific™ Dilution Buffer for Restriction Enzymes

Optimize applications that require diluted enzymes with this dilution buffer.

Pricing and Availability

Thermo Scientific™ EcoRI (10 U/μL)

The EcoRI restriction enzyme recognizes G^AATTC sites and cuts best at 37°C in its own unique buffer.

Pricing and Availability
Specifications

Concentration	10 U/μL

Thermo Scientific™ AarI (2 U/μL)

Cut at CACCTGC(4/8)^ sites with AarI restriction enzyme, which performs best at 37°C in its own unique (+oligo) buffer.

Pricing and Availability
Specifications

Sensitive to Heat Inactivation	Yes
Product Type	Restriction Enzyme
Enzyme	AarI
Methylation Sensitivity	CpG Methylation-Sensitive,Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive
Research Category	Traditional Cloning
Compatible Buffer	Unique Buffer (10X Buffer AarI)
Type IIS RE	No
Optimal Reaction Temperature	37°C
Concentration	2 U/μL

Thermo Scientific™ DpnI (10 U/μL)

Cut at Gm6A^TC sites with DpnI restriction enzyme, which performs best at 37°C in Tango buffer (Isoschizomers: MalI).

Pricing and Availability
Specifications

Concentration	10 U/μL

Thermo Scientific™ FastDigest™ NheI: PROMO

Thermo Scientific FastDigest NheI restriction enzyme recognizes G^CTAGC site and cuts best at 37°C in 5 to 15 minutes using universal FastDigest Buffer. Isoschizomers: AsuNHI, BmtI.

Pricing and Availability

Thermo Scientific™ Eco81I (Bsu36I) (10 U/μL)

The Eco81I (Bsu36I) restriction enzyme recognizes CC^TNAGG sites and cuts best at 37°C in Tango buffer (Isoschizomers: AxyI, Bse21I, Bsu36I).

Pricing and Availability
Specifications

Sensitive to Heat Inactivation	Yes
Product Type	Restriction Enzyme
Enzyme	Eco81I (Bsu36I)
Methylation Sensitivity	Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive,Not CpG Methylation-Sensitive
Research Category	Traditional Cloning
Compatible Buffer	10X Buffer Tango
Type IIS RE	No
Optimal Reaction Temperature	37°C
Concentration	10 U/μL

Thermo Scientific™ BglII (10 U/μL)

Cut at A^GATCT sites with BglII restriction enzyme, which performs best at 37°C in O buffer.

Pricing and Availability
Specifications

Concentration	10 U/μL

Thermo Scientific™ PvuII (10 U/μL)

The PvuII restriction enzyme recognizes CAG^CTG sites and cuts best at 37°C in G buffer.

Pricing and Availability
Specifications

Concentration	10 U/μL

Thermo Scientific™ RsaI (10 U/μL)

The RsaI restriction enzyme recognizes GT^AC sites and cuts best at 37°C in Tango buffer (Isoschizomers: AfaI, CviQI, RsaNI).

Pricing and Availability
Specifications

Sensitive to Heat Inactivation	Yes
Product Type	Restriction Enzyme
Enzyme	Rsa I
Methylation Sensitivity	CpG Methylation-Sensitive,Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive
Research Category	Traditional Cloning
Compatible Buffer	10X Buffer Tango
Type IIS RE	No
Optimal Reaction Temperature	37°C
Concentration	10 U/μL

Thermo Scientific™ PaeI (SphI) (10 U/μL)

The PaeI (SphI) restriction enzyme recognizes GCATG^C sites and cuts best at 37°C in B buffer (Isoschizomers: BbuI, SphI).

Pricing and Availability
Specifications

Sensitive to Heat Inactivation	Yes
Product Type	Restriction Enzyme
Enzyme	PaeI (SphI)
Methylation Sensitivity	Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive,Not CpG Methylation-Sensitive
Research Category	Traditional Cloning
Compatible Buffer	10X Buffer B
Type IIS RE	No
Optimal Reaction Temperature	37°C
Concentration	10 U/μL

Thermo Scientific™ HindIII (10 U/μL)

The HindIII restriction enzyme recognizes A^AGCTT sites and cuts best at 37°C in R buffer.

Pricing and Availability
Specifications

Concentration	10 U/μL

Thermo Scientific™ ApaI (10 U/μL)

Cut at GGGCC^C sites with ApaI restriction enzyme, which functions best at 37°C in B buffer (Isoschizomers: PspOMI).

Pricing and Availability
Specifications

Concentration	10 U/μL

Thermo Scientific™ Tru1I (MseI) (10 U/μL)

Cut at T^TAA sites with Tru1I (MseI) restriction enzyme, which performs best at 65°C in R buffer (Isoschizomers: MseI, Tru9I).

Pricing and Availability
Specifications

Sensitive to Heat Inactivation	No
Product Type	Restriction Enzyme
Enzyme	Tru1I (MseI)
Methylation Sensitivity	Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive,Not CpG Methylation-Sensitive
Research Category	Traditional Cloning
Compatible Buffer	10X Buffer R
Type IIS RE	No
Optimal Reaction Temperature	65°C
Concentration	10 U/μL

Restriction Enzymes

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