Chromatography Media
Chromatography Media
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Filtered Search Results
Applied Biosystems™ Trifluoroacetic Acid (TFA) Neat Liquid
Trifluoroacetic Acid (TFA, concentrated) for use with OPC™ cartridges for oligonucleotide purification.
Cytiva Heparin Sepharose™ 6 Fast Flow Affinity Chromatography Media
USe for separations of biomolecules with an affinity for heparin, including antithrombin III, coagulation factors and other plasma proteins. Cytiva Life Sciences™ Heparin Sepharose™ 6 Fast Flow Affinity Chromatography Media is fast and reliable and highly suitable for process-scale purification processes.
Cytiva Strep-Tactin™ XT Sepharose™ Chromatography Resin
Chromatography resin for purification of recombinant proteins tagged with a Strep-tag™ II and Twin-Strep-tag™.
Particle Size | 34 μm |
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Ligand Type | Strep-Tactin XT |
Density (Metric) | Approx. 5 mg/mL resin |
Matrix | Rigid, cross-linked agarose, spherical |
Stability | Stable in all commonly used aqueous buffers |
pH Range | 6 to 10 pH |
Binding Capacity | Approx. 10 mg protein with Strep-tag II or Twin-Strep-tag/mL of resin |
Storage Requirements | 2 to 8°C in 20% ethanol |
For Use With (Application) | Purification of recombinant proteins tagged with Strep-tag II and Twin-Strep-tag |
Cytiva HiTrap™ TALON™ Crude Prepacked Columns
For purification of histidine-tagged recombinant proteins by immobilized metal affinity chromatography (IMAC). Cytiva Lifescience™ HiTrap™ TALON™ Crude Prepacked Columns offer a cobalt-based medium allowing protein purification under native or denaturing conditions.
Particle Size | 60 to 160 μm |
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Ligand Type | Talon Superflow Cobalt-IMAC |
Flow Rate | 1 to 4 mL/min |
Matrix | 6% Cross-Linked Agarose |
Storage Buffer | 20% Ethanol |
pH Range | 3 to 12 |
Product Type | HiTrap Talon crude prepacked Column |
Material | Polypropylene |
Storage Requirements | 4°C to 8°C |
For Use With (Application) | For PurifiCatIon of Histidine-Tagged Recombinant Proteins by Immobilized Metal Affinity Chromatography, Used Buffers and Easily Regenerated with 0.5 M NaOH |
Max. Pressure | 3 bar |
Cytiva Capto™ Adhere Media
A multimodal anion exchanger to follow the Protein A capture step, making for a streamlined process
Cytiva Capto™ Multimodal (MMC) Chromatography Media
Used to solve specific purification challenges at both high and low ionic strengths
Particle Size | 75 μm |
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Product Type | Capto MMC Media |
Cytiva TALON™ Superflow Medium
Offers different selectivity compared to nickel-charged media. Cytiva TALON™ Superflow Medium used for purification of histidine-tagged recombinant proteins by IMAC.
Particle Size | 60 to 160 μm |
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Ligand Type | Talon Superflow Cobalt-IMAC |
Matrix | 6% Cross-Linked Agarose |
Storage Buffer | 20% Ethanol |
pH Range | 3 to 12 |
Product Type | Talon Superflow Media |
Binding Capacity | Up to 20 mg Histidine-tagged Protein |
Storage Requirements | 4°C to 8°C |
For Use With (Application) | For PurifiCatIon of Histidine-Tagged Recombinant Pby Immobilized Metal Affinity Chromatography |
Cytiva Blue Sepharose™ 6 Fast Flow Chromatography Media
Blue dye binds to proteins such as albumin and lipoproteins as well as enzymes like kinases and dehydrogenases
Cytiva Butyl-S Sepharose™ 6 Fast Flow Chromatography Media
The least hydrophobic medium in the Sepharose Fast Flow hydrophobic interaction chromatography (HIC) range and is particularly useful during the initial stages of purification process
Cytiva nProtein A Sepharose™ 4 Fast Flow Affinity Media
Used for purification of monoclonal and polyclonal antibodies. Cytiva Lifescience™ nProtein A Sepharose™ 4 Fast Flow Affinity Media contains native protein A coupled to Sepharose 4 Fast Flow.
Particle Size | 90 μm |
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Ligand Type | Native Protein A (S. aureus) |
Flow Rate | 150 to 250 cm/Hoμr |
Matrix | 4% Cross-Linked Agarose |
Stability | No significant change in chromatographic performance after 1 week storage using 8 M Urea, 6 M Gua-HCI, 2% Benzyl Alcohol or 20% Ethanol |
Storage Buffer | 20% Ethanol |
Product Type | nProtein A Sepharose 4 Fast Flow Media |
Binding Capacity | >35 mg Human IgG/mL |
Height (English) Bed | 9.84 in. |
Storage Requirements | 2°C to 8°C |
Height (Metric) Bed | 25 cm |
Max. Pressure | 100 kPa |
Cytiva Butyl Sepharose™ High Performance Chromatography Media
Designed for intermediate and polishing step purification steps
Particle Size | 24 to 44 μm |
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Ligand Type | Butyl |
Flow Rate | 100 to 200 cm/Hoμr |
Matrix | Cross-Linked Agarose |
Stability | Stable in commonly used aqueous Buffers: 1 M Sodium Hydroxide, 1 M Acetic Acid, 8 M Urea, 6 M Guanidine Hydrochloride, 30% Acetonitrile, 70% Ethanol, 3 M Ammonium sulfate, 1 mM HCl, 30% Isopropanol, 2% SDS |
Storage Buffer | 20% Ethanol |
pH Range | 3 to 13 |
Product Type | Butyl Sepharose High Performance Media |
Binding Capacity | 38 mg b-lactoglobulin/mL mediμm |
Storage Requirements | 4°C to 30°C |
For Use With (Application) | For Intermediate and Polishing Step PurifiCatIon Steps |
Max. Pressure | 300 kPa |
Cytiva DEAE Sepharose™ Fast Flow Chromatography Media
For preparative protein separations in both research and industrial applications. Cytiva Life Sciences™ DEAE Sepharose™ Fast Flow Chromatography Media can be used for capture and intermediate purification of wide range of approved biopharmaceuticals.
Cytiva His MultiTrap™ TALON™
For high-throughput screening of histidine-tagged proteins from clarified cell lysates
Cytiva CNBr-Activated Sepharose™ 4 Fast Flow Affinity Media
Used for coupling of large amino-containing ligands