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DMEM-F-12 Cell Culture Media

DMEM/F-12 cell culture media is a serum-free formulation that combines Dulbecco's modified Eagle medium (DMEM) and Ham's F-12 nutrient mixture in a 1:1 ratio. It is designed to provide a suitable environment for the growth and maintenance of various cell types in laboratory settings. This medium is enriched with essential nutrients, including insulin, transferrin, and growth factors, supporting the optimal proliferation and survival of cells1. DMEM/F-12 cell culture media has been widely used for culturing primary mammalian cells as well as various normal and transformed cell lines. Its versatility and ability to support a broad range of cell types make it a valuable tool in cellular research and biotechnological applications.
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1
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Gibco™ DMEM/F-12, GlutaMAX™ Supplement

DMEM/F-12, GlutaMAX™ Supplement

Content And Storage 2 to 8°C
Description DMEM/F-12 Medium, 500mL, 1 X Concentration, Liquid Form, With GlutaMAX™, Phenol Red, Sodium Pyruvate, 6.8 to 7.2 pH, 12 Months Shelf Life, 290 to 330mOsm/kg Osmolality, 2 to 8°C Storage Conditions, Used for Mammalian Cell Culture
With Additives GlutaMAX,Phenol Red,Sodium Pyruvate
Product Type DMEM/F-12 Medium
Form Liquid
Without Additives No HEPES
pH 6.8 to 7.2
Certifications/Compliance ISO 13485 standards, FDA
Shelf Life 12 Months
Concentration 1 X
For Use With (Application) Mammalian cell culture
2
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Gibco™ DMEM/F-12

DMEM/F-12 (Dulbecco's Modified Eagle Medium/Nutrient Mixture F-12) is a widely used basal medium for supporting the growth of many different mammalian cells.

Shipping Condition Room Temperature
Cell Line MDCK, glial cells, fibroblasts, human endothelial cells, and rat fibroblasts
Content And Storage Storage conditions: 2°C to 8°C (protect from light)
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture
Form Liquid
Manufacturing Quality cGMP-compliant under the ISO 13485 standard
Concentration 1 X
Sterility Sterile-filtered
Cell Type Primary Fibroblasts, Neurons, Glial Cells, HUVECs, Smooth Muscle Cells
Sterilization Method Sterile-filtered
With Additives High Glucose,Glutamine,Phenol Red,Sodium Pyruvate
Product Type DMEM (Dulbecco's Modified Eagle Medium)/F-12
Classification Animal Origin-free
Shelf Life 12 Months From Date of Manufacture
Product Line Gibco
Serum Level Standard Serum Supplementation
3
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Gibco™ Advanced DMEM/F-12

Advanced DMEM/F-12 (Dulbecco's Modified Eagle Medium/Ham's F-12) is a widely used basal medium that allows the culture of mammalian cells with reduced Fetal Bovine Serum (FBS) supplementation.

Shipping Condition Room Temperature
Cell Line MRC-5, SP2, Vero, WI-38 and Jurkat
Content And Storage Storage conditions: 2-8° C. Protect from light
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture
Form Liquid
Without Additives No Glutamine,No HEPES
Manufacturing Quality cGMP-compliant under the ISO 13485 standard
Concentration 1 X
Sterility Sterile-filtered
Sterilization Method Sterile-filtered
With Additives High Glucose,Phenol Red,Sodium Pyruvate,Non Essential Amino Acids (NEAA)
Product Type DMEM (Dulbecco's Modified Eagle Medium)/F-12
Classification Animal Origin
Shelf Life 12 Months From Date of Manufacture
Product Line Gibco
Serum Level Reduced Serum
4
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Gibco™ DMEM/F-12, GlutaMAX™ supplement

DMEM/F-12 (Dulbecco's Modified Eagle Medium/Nutrient Mixture F-12) is a widely used basal medium for supporting the growth of many different mammalian cells.

Sterilization Method Sterile-filtered
With Additives High Glucose,GlutaMAX,Phenol Red,Sodium Pyruvate
Product Type DMEM (Dulbecco's Modified Eagle Medium)/F-12
Form Liquid
Without Additives No HEPES
Classification Animal Origin-free
Manufacturing Quality cGMP-compliant under the ISO 13485 standard
Concentration 1 X
Sterility Sterile-filtered
5
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Gibco™ SILAC Advanced DMEM/F-12 Flex Media, no glucose, no phenol red

SILAC Advanced DMEM/F-12 Flex Media, no glucose, no phenol red

EDGE
6
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Gibco™ DMEM/F-12, HEPES

Dulbecco's Modified Eagle Medium:Nutrient Mixture F-12 (DMEM / F-12) is a widely used basal medium for supporting the growth of many different mammalian cells.

Sterilization Method Sterile-filtered
With Additives High Glucose,Glutamine,HEPES,Phenol Red,Sodium Pyruvate
Product Type DMEM (Dulbecco's Modified Eagle Medium)/F-12
Form Liquid
Classification Animal Origin-free
Manufacturing Quality cGMP-compliant under the ISO 13485 standard
Concentration 1 X
Sterility Sterile-filtered
7
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Gibco™ DMEM/F-12, no glutamine

Dulbecco's Modified Eagle Medium:Nutrient Mixture F-12 (DMEM/F-12) is a widely used basal medium for supporting the growth of many different mammalian cells.

Form Liquid
Without Additives No Glutamine,No HEPES
Manufacturing Quality cGMP-compliant under the ISO 13485 standard
Shelf Life 12 Months
8
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Gibco™ DMEM/F-12, HEPES, no phenol red

DMEM/F-12 (Dulbecco's Modified Eagle Medium/Nutrient Mixture F-12) is a widely used basal medium for supporting the growth of many different mammalian cells.

Cell Line MDCK, glial cells, fibroblasts, human endothelial cells, and rat fibroblasts
Content And Storage Storage conditions: 2°C to 8°C (protect from light)
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture
Form Liquid
Without Additives No Phenol Red,No Sodium Pyruvate
Manufacturing Quality cGMP-compliant under the ISO 13485 standard
Concentration 1X
Sterility Sterile-filtered
Sterilization Method Sterile-filtered
With Additives High Glucose,Glutamine,HEPES
Product Type DMEM (Dulbecco's Modified Eagle Medium)/F-12
Classification Animal Origin-free
Shelf Life 12 Months From Date of Manufacture
Product Line Gibco
Serum Level Standard Serum Supplementation
9

Gibco™ DMEM/F-12, HEPES

DMEM/F-12, HEPES

Regulatory Status In-vitro Diagnostic Use
Content And Storage 2 to 8°C
Description DMEM/F-12 Medium, 500mL, 1 X Concentration, Liquid Form, With L-glutamine, HEPES, Phenol Red, Sodium Pyruvate, 6.9 to 7.3 pH, 12 Months Shelf Life, 270 to 330mOsm/kg Osmolality, 2 to 8°C Storage Conditions, Used for Mammalian Cell Culture
With Additives Glutamine,HEPES,Phenol Red,Sodium Pyruvate
Product Type DMEM/F-12 Medium
Form Liquid
pH 6.9 to 7.3
Certifications/Compliance ISO 13485 standards, FDA
Shelf Life 12 Months
Concentration 1 X
For Use With (Application) Mammalian cell culture
10

Gibco™ DMEM/F-12, powder

DMEM/F-12, powder

Form Powder
Without Additives No HEPES,No Sodium Bicarbonate
pH 6.2 to 6.6
Certifications/Compliance ISO 13485 standards, FDA
Shelf Life 36 Months
For Use With (Application) Mammalian cell culture
11
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Gibco™ DMEM/F-12, no phenol red

DMEM/F-12 (Dulbecco's Modified Eagle Medium/Nutrient Mixture F-12) is a widely used basal medium for supporting the growth of many different mammalian cells.

12
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Gibco™ DMEM/F-12

DMEM/F-12

13
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Corning™ cellgro™ DMEM/Ham's Medium F-12 Mix
BIOPHARMA

Used for carcinoma cells, rat skeletal myoblasts, Chinese hamster lung cells, and rat, rabbit, and chicken embryos. Corning™ cellgro™ DMEM/Ham's Medium F-12 Mix is based on Ham's F-10 medium with increased concentrations of choline, inositol, putrescine, and several amino acids.

14
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Cytiva HyClone™ DMEM/F12 1:1 Media

Used for culturing many different mammalian cells including MDCK, Glial cells, fibroblasts, and endothelial cells. HyClone™ DMEM/F12 1:1 Media supports cell culture growth and biomanufacturing processes and is manufactured using ISO 9001:2000-certified processes.

Content And Storage 2° to 8°C, away from light
Sterilization Method 0.1 μm Sterile-filtered
Product Type DMEM/F12 1:1 Medium
Form Liquid
Certifications/Compliance ISO 9001:2000
Shelf Life 12 Months
Sterility Sterile-filtered
15
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Gibco™ CTS™ KnockOut™ DMEM/F-12

CTS KnockOut DMEM/F-12 is a serum free, low osmolality medium without L-glutamine or HEPES buffer that is optimized for the growth of human embryonic stem cells (ESC) and induced pluripotent stem cells (iPSC).