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Invitrogen™ SuperScript™ IV First-Strand Synthesis System
The SuperScript IV First-Strand Synthesis System for RT-PCR, available with or without ezDNase, is optimized for synthesis of first-strand cDNA from purified poly(A)+ or total RNA.
| GC-Rich PCR Performance | High |
|---|---|
| Format | Kit |
| Reverse Transcriptase | SuperScript IV |
| Size (Final Product) | Up to 12.3 kb |
| Reaction Format | Separate components |
| Final Product Type | First-Strand cDNA |
| Reaction Speed | 10 min. |
| Reagent Type | Reverse Transcription |
| Quantity | Each |
| Optimal Reaction Temperature | 50°C |
| Technique | Reverse Transcription |
| Starting Material | RNA |
Applied Biosystems™ MicroAmp™ Fast Optical 48-Well Reaction Plate
Optimized to provide unmatched temperature accuracy and uniformity for fast, efficient PCR amplification
Applied Biosystems™ MicroAmp™ EnduraPlate™ Optical 96-Well Clear Reaction Plates with Barcode
Ideal solution for work that requires special handling and an even greater degree of durability for use with multi-instrument experiments
| Skirt Style | Semi |
|---|---|
| Content And Storage | Store at room temperature. |
| No. of Wells | 96 |
| For Use With (Equipment) | Thermal Cycler, Genetic Analyzer, DNA Analyzer |
| Product Type | Microplate |
| Reaction Speed | Standard |
| Volume (Metric) Working | 0.2 mL |
| Barcode | Includes Barcode |
| Color | Clear,Optical |
| Material | Polycarbonate,Polypropylene |
| Certifications/Compliance | Certified DNA/RNase Free |
| Volume (Metric) Well | 0.3 mL |
| Product Line | MicroAmp |
Invitrogen™ Platinum™ SuperFi™ DNA Polymerase
Invitrogen Platinum SuperFi DNA Polymerase is a proofreading DNA polymerase that combines superior fidelity with trusted Platinum hot-start technology for the highest success in PCR.
| Shipping Condition | Wet or Dry Ice |
|---|---|
| Polymerase | Platinum SuperFi DNA Polymerase |
| GC-Rich PCR Performance | High |
| Fidelity (vs. Taq) | 300X |
| Size (Final Product) | 20 kb or less |
| Reaction Format | Separate Components |
| Reaction Speed | Fast |
| Hot Start | Built-In Hot Start |
| Overhang | Blunt |
| For Use With (Application) | Hot-start PCR,High-fidelity PCR |
Applied Biosystems™ MicroAmp™ 48-Well Optical Adhesive Film
Reduces the chance of well-to-well contamination and sample evaporation when applied to a 48-well microplate
| Plate Compatibility | 48-well Plate |
|---|---|
| Shipping Condition | Room Temperature |
| No. of Wells | 48 |
| Product Type | Film, Seal, or Applicator |
| For Use With (Equipment) | StepOne™, |
| Color | Optical |
| Material | Polyester |
| Adhesive | Pressure Sensitive |
| Product Line | MicroAmp |
Applied Biosystems™ MicroAmp™ 8-Cap Strip, clear
Designed to fit on MicroAmp reaction tubes, tube strips, and 96-well plates
| No. of Wells | 96 |
|---|---|
| Product Type | 8-Strip Cap |
| For Use With (Equipment) | Thermal Cyclers |
| Color | Natural |
| Material | Polypropylene |
| Certifications/Compliance | Certified DNA/Rnase Free |
| Closure Type | Domed Cap |
| Product Line | MicroAmp™ |
Invitrogen™ SuperScript™ IV One-Step RT-PCR System
The Invitrogen SuperScript One-Step RT-PCR System, available with or without ezDNase, combines the high processivity of SuperScript IV Reverse Transcriptase (RT) with the high-fidelity of Platinum SuperFi DNA Polymerase to provide unmatched one-step RT-PCR performance.
| Shipping Condition | Dry Ice |
|---|---|
| GC-Rich PCR Performance | High |
| Reverse Transcriptase | SuperScript IV |
| Final Product Type | PCR Amplified cDNA |
| Quantity | Each |
| PCR Method | 1-step RT-PCR |
| Detection Method | Gel Electrophoresis |
| Polymerase | Platinum SuperFi |
| Format | Kit |
| Size (Final Product) | Up to 14 kb |
| Reaction Format | Master Mix |
| Reaction Speed | 10 min. |
| Reagent Type | Reverse Transcription |
| Ribonuclease H Activity | Reduced |
| Hot Start | Built-In Hot Start |
| Optimal Reaction Temperature | 45°C to 60°C |
| Technique | 1-step RT-PCR |
| Starting Material | RNA |
Invitrogen™ Taq DNA Polymerase, native
Taq DNA Polymerase is a thermostable enzyme that synthesizes DNA from single-stranded templates in the presence of dNTPs and a primer.
| Shipping Condition | Wet or Dry Ice |
|---|---|
| GC-Rich PCR Performance | Low |
| Fidelity (vs. Taq) | 1X |
| Overhang | 3'-A |
| Concentration | 5 U/μL |
| For Use With (Application) | Standard PCR |
| Exonuclease Activity | 5' - 3' |
| Polymerase | Taq DNA Polymerase |
| Format | Stand-alone enzyme |
| Size (Final Product) | 5 kb or less |
| Reaction Format | Separate Components |
| Product Type | DNA Polymerase |
| Reaction Speed | Standard |
| Hot Start | No |
| Starting Material | DNA |
Invitrogen™ Taq DNA Polymerase, recombinant
Taq DNA Polymerase is a thermostable enzyme that synthesizes DNA from single-stranded templates in the presence of dNTPs and a primer.
| Shipping Condition | Dry Ice |
|---|---|
| GC-Rich PCR Performance | Low |
| Fidelity (vs. Taq) | 1X |
| Overhang | 3'-A |
| Concentration | 5 U/μL |
| For Use With (Application) | Standard PCR |
| PCR Method | qPCR,Standard PCR |
| Exonuclease Activity | 5' - 3' |
| Polymerase | Taq DNA Polymerase |
| Format | Stand-alone enzyme |
| Size (Final Product) | 5 kb or less |
| Reaction Format | Separate Components |
| Product Type | DNA Polymerase |
| Reaction Speed | Standard |
| Hot Start | No |
| Starting Material | DNA |
Applied Biosystems™ MicroAmp™ Optical Adhesive Film
Reduces chance of well-to-well contamination and sample evaporation when applied to a microplate
| Plate Compatibility | 96/384-well Plate |
|---|---|
| Shipping Condition | Room Temperature |
| Product Type | Film, Seal, or Applicator |
| For Use With (Equipment) | 2720 Thermal Cycler, 6100 Thermal Cycler, 7000 System, 7300 System, 7500 Fast System, 7500 System, 7900HT Fast System, 7900HT System, GeneAmp 9700, QuantStudio™, SimpliAmp™ Thermal Cycler, StepOnePlus™, Veriti Dx Fast Thermal Cycler, Veriti Dx Thermal Cycler, Veriti Fast Thermal Cycler, Veriti Thermal Cycler, ViiA™ 7 Dx System, ViiA™ 7 System, ViiA™ 7 Dx Fast System, QuantStudio™ Dx, 7500 Fast Dx System, ViiA™ 7 Fast System, ProFlex™ PCR System |
| Color | Optical |
| Material | Polyester |
| Adhesive | Pressure Sensitive |
| Product Line | MicroAmp |
Applied Biosystems™ MicroAmp™ Fast Optical 96-Well Reaction Plate, 0.1 mL
Reduces PCR reaction time from 2 hours to as little as 25 minutes
Invitrogen™ SuperScript™ IV Reverse Transcriptase
SuperScript IV Reverse Transcriptase (RT) is a proprietary MMLV mutant with superior robustness and reliability in RT reactions.
| Shipping Condition | Dry Ice |
|---|---|
| GC-Rich PCR Performance | High |
| Reverse Transcriptase | SuperScript IV |
| Final Product Type | First-Strand cDNA |
| Concentration | 200 U/μL |
| Format | Stand-alone Enzyme |
| Size (Final Product) | Up to 12.3 kb |
| Reaction Format | Separate components |
| Reaction Speed | 10 min. |
| Reagent Type | Reverse Transcription |
| Ribonuclease H Activity | Reduced |
| Optimal Reaction Temperature | 50°C |
| Technique | Reverse Transcription |
| Starting Material | RNA |
Applied Biosystems™ MicroAmp™ Optical 8-Cap Strips
Tight seal minimizes evaporation and contamination
Applied Biosystems™ MicroAmp™ Clear Adhesive Film
Creates a secure seal across entire microplate to prevent evaporation and well-to-well contamination.
Applied Biosystems™ MicroAmp™ Fast Optical 96-Well Reaction Plate with Barcode, 0.1 mL
Reduces PCR reaction time from 2 hours to as little as 25 minutes
| Skirt Style | Semi |
|---|---|
| No. of Wells | 96 |
| For Use With (Equipment) | Thermal Cycler, Genetic Analyzer, DNA Analyzer |
| Product Type | Microplate |
| Reaction Speed | Fast |
| Volume (Metric) Working | 0.1 mL |
| Barcode | Includes Barcode |
| Color | Optical |
| Material | Polypropylene |
| Certifications/Compliance | Certified DNA/RNase Free |
| Volume (Metric) Well | 0.2 mL |
| Product Line | MicroAmp |