Filtered Search Results
Thermo Scientific™ EcoRI (10 U/μL)
The EcoRI restriction enzyme recognizes G^AATTC sites and cuts best at 37°C in its own unique buffer.
| Concentration | 10 U/μL |
|---|
Thermo Scientific™ T4 DNA Ligase, HC (30 U/μL)
T4 DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5'-phosphate and 3'-hydroxyl termini in duplex DNA or RNA.
Thermo Scientific™ AarI (2 U/μL)
Cut at CACCTGC(4/8)^ sites with AarI restriction enzyme, which performs best at 37°C in its own unique (+oligo) buffer.
| Sensitive to Heat Inactivation | Yes |
|---|---|
| Product Type | Restriction Enzyme |
| Enzyme | AarI |
| Methylation Sensitivity | CpG Methylation-Sensitive,Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive |
| Research Category | Traditional Cloning |
| Compatible Buffer | Unique Buffer (10X Buffer AarI) |
| Type IIS RE | No |
| Optimal Reaction Temperature | 37°C |
| Concentration | 2 U/μL |
Invitrogen™ RNase Cocktail™ Enzyme Mix
Endonuclease-free, exonuclease-free, protease-free
| Shipping Condition | Dry Ice |
|---|---|
| Product Type | RNAse Cocktail Enzyme Mix |
| Enzyme | RNase |
| Product Line | Ambion™ |
Thermo Scientific™ S1 Nuclease (100 U/μL)
Degrade single-stranded DNA and RNA with this endonuclease.
Invitrogen™ T4 DNA Ligase (1 U/μL)
Ideal for cloning (blunt-end or cohesive-end ligation) and adding linkers or adapters to blunt-ended DNA
| Product Type | T4 DNA Ligase |
|---|---|
| Enzyme | Ligase |
| Concentration | 1 U/μL |
Thermo Scientific™ Klenow Fragment, exo– (5 U/μL)
Klenow Fragment, exo- is the large fragment of DNA polymerase I. Exhibits 5'→3' polymerase activity, but lacks the 3'→5' and 5'→3' exonuclease activities.
| Polymerase | DNA Polymerase I |
|---|---|
| Product Type | Klenow Fragment |
| Compatible Buffer | Restriction Enzyme, PCR, RT Buffer |
| Concentration | 5 U/μL |
Thermo Scientific™ HincII (HindII) (10 U/μL)
The HincII (HindII) restriction enzyme recognizes GTY^RAC sites and cuts best at 37°C in Tango buffer (Isoschizomers: HindII).
| Sensitive to Heat Inactivation | Yes |
|---|---|
| Product Type | Restriction Enzyme |
| Enzyme | HincII (HindII) |
| Methylation Sensitivity | CpG Methylation-Sensitive,Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive |
| Research Category | Traditional Cloning |
| Compatible Buffer | 10X Buffer Tango |
| Type IIS RE | No |
| Optimal Reaction Temperature | 37°C |
| Concentration | 10 U/μL |
Thermo Scientific™ PstI (10 U/μL)
The PstI restriction enzyme recognizes CTGCA^G sites and cuts best at 37°C in O buffer (Isoschizomers: BspMAI).
| Concentration | 10 U/μL |
|---|
Thermo Scientific™ DraI (10 U/μL)
Thermo Scientific™ DraI is available as a FastDigest™ enzyme which is used for rapid DNA digestion.
Invitrogen™ T4 DNA Ligase (5 U/μL)
Catalyzes the formation of phosphodiester bonds in the presence of ATP between double-stranded DNAs with 3' hydroxyl and 5' phosphate termini
Thermo Scientific™ Uracil-DNA Glycosylase (1 U/μL)
Uracil-DNA Glycosylase (UDG, UNG) catalyzes the hydrolysis of the N-glycosylic bond between uracil and sugar in DNA and prevents carry-over of DNA in PCR reactions.
| Content And Storage | Uracil-DNA glycolase (1 U/μL) and 10X Reaction Buffer. Store at -20°C. |
|---|---|
| Product Type | Uracil DNA Glycosylase |
| Enzyme | Uracil-DNA Glycosylase |
| Compatible Buffer | 10X Reaction Buffer |
| Concentration | 1 U/μL |
Thermo Scientific™ PscI (PciI) (10 U/μL)
The PscI (PciI) restriction enzyme recognizes A^CATGT sites and cuts best at 37°C in Tango buffer (Isoschizomers: PciI).
| Sensitive to Heat Inactivation | Yes |
|---|---|
| Product Type | Restriction Enzyme |
| Enzyme | PscI (PciI) |
| Methylation Sensitivity | Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive,Not CpG Methylation-Sensitive |
| Research Category | Traditional Cloning |
| Compatible Buffer | 10X Buffer Tango |
| Type IIS RE | No |
| Optimal Reaction Temperature | 37°C |
| Concentration | 10 U/μL |
Thermo Scientific™ NdeI (10 U/μL)
The NdeI restriction enzyme recognizes CA^TATG sites and cuts best at 37°C in O buffer (Isoschizomers: FauNDI).
| Concentration | 10 U/μL |
|---|
Thermo Scientific™ FastDigest AatII
Cut at GACGT^C sites with Thermo Scientific™ FastDigest™ AatII, which performs best at 37°C in 5-15 minutes using universal FastDigest™ Buffer (Isoschizomers: ZraI).