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Thermo Scientific™ T4 DNA Ligase, HC (30 U/μL)
T4 DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5'-phosphate and 3'-hydroxyl termini in duplex DNA or RNA.
Thermo Scientific™ FastDigest NcoI
The FastDigest NcoI restriction enzyme recognizes C^CATGG sites and cuts best at 37°C in 5-15 minutes using universal FastDigest Buffer (Isoschizomers: Bsp19I).
| Sensitive to Heat Inactivation | Yes |
|---|---|
| Product Type | Restriction Enzyme |
| Enzyme | Nco I |
| Methylation Sensitivity | Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive,Not CpG Methylation-Sensitive |
| Research Category | Traditional Cloning |
| Compatible Buffer | 10X FastDigest Buffer/FastDigest Green Buffer |
| Overhang | 5' protruding ends |
| Type IIS RE | No |
| Optimal Reaction Temperature | 37°C |
| Inactivation Temperature | 65°C |
| Product Line | FastDigest™ |
Thermo Scientific™ AarI (2 U/μL)
Cut at CACCTGC(4/8)^ sites with AarI restriction enzyme, which performs best at 37°C in its own unique (+oligo) buffer.
| Sensitive to Heat Inactivation | Yes |
|---|---|
| Product Type | Restriction Enzyme |
| Enzyme | AarI |
| Methylation Sensitivity | CpG Methylation-Sensitive,Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive |
| Research Category | Traditional Cloning |
| Compatible Buffer | Unique Buffer (10X Buffer AarI) |
| Type IIS RE | No |
| Optimal Reaction Temperature | 37°C |
| Concentration | 2 U/μL |
Thermo Scientific™ RNase I (10 U/μL)
Ribonuclease I (RNase I) is an endoribonuclease that hydrolyzes single-stranded RNA to nucleoside 3'-monophosphates.
| Product Type | RNase I |
|---|---|
| Enzyme | RNase |
| Concentration | 10 U/μL |
Thermo Scientific™ NTP Set, 100 mM Solution
The Thermo Scientific NTP set is a convenient set of 100 mM solutions of each ATP, CTP, GTP and UTP.
Thermo Scientific™ FastDigest SacI
The FastDigest SacI restriction enzyme recognizes GAGCT^C sites and cuts best at 37°C in 5-15 minutes using universal FastDigest Buffer (Isoschizomers: Eco53kI, EcoICRI, Psp124BI, SstI).
| Sensitive to Heat Inactivation | Yes |
|---|---|
| Product Type | Restriction Enzyme |
| Enzyme | SacI |
| Methylation Sensitivity | Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive,Not CpG Methylation-Sensitive |
| Research Category | Traditional Cloning |
| Compatible Buffer | 10X FastDigest Buffer/FastDigest Green Buffer |
| Overhang | 3' protruding ends |
| Type IIS RE | No |
| Optimal Reaction Temperature | 37°C |
| Inactivation Temperature | 65°C |
| Product Line | FastDigest™ |
Thermo Scientific™ NcoI (10 U/μL)
The NcoI restriction enzyme recognizes C^CATGG sites and cuts best at 37°C in Tango buffer (Isoschizomers: Bsp19I).
| Concentration | 10 U/μL |
|---|
Thermo Scientific™ FastDigest BglII
Cut at A^GATCT sites with FastDigest™ BglII restriction enzyme, which performs best at 37°C in 5-15 minutes using universal FastDigest Buffer.
| Sensitive to Heat Inactivation | No |
|---|---|
| Product Type | Restriction Enzyme |
| Enzyme | Bgl II |
| Methylation Sensitivity | Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive,Not CpG Methylation-Sensitive |
| Research Category | Traditional Cloning |
| Compatible Buffer | 10X FastDigest Buffer/FastDigest Green Buffer |
| Overhang | 5' protruding ends |
| Type IIS RE | No |
| Optimal Reaction Temperature | 37°C |
| Product Line | FastDigest™ |
Thermo Scientific™ Klenow Fragment, exo– (5 U/μL)
Klenow Fragment, exo- is the large fragment of DNA polymerase I. Exhibits 5'→3' polymerase activity, but lacks the 3'→5' and 5'→3' exonuclease activities.
| Polymerase | DNA Polymerase I |
|---|---|
| Product Type | Klenow Fragment |
| Compatible Buffer | Restriction Enzyme, PCR, RT Buffer |
| Concentration | 5 U/μL |
Thermo Scientific™ DNase I, RNase-free, HC (50 U/μL)
Endonuclease that nonspecifically cleaves single- and double-stranded DNA to release di-, tri-, and oligonucleotides with 5'-phosphate and 3'-OH groups.
Thermo Scientific™ PstI (10 U/μL)
The PstI restriction enzyme recognizes CTGCA^G sites and cuts best at 37°C in O buffer (Isoschizomers: BspMAI).
| Concentration | 10 U/μL |
|---|
Invitrogen™ ezDNase™ Enzyme
SuperScript™ IV VILO™ Master Mix is a reaction master mix designed for fast, sensitive, and reproducible cDNA synthesis in RT-qPCR applications.
Invitrogen™ UltraPure™ Glycogen
Used as carrier for nucleic acid precipitation in ethanol
Thermo Scientific™ SalI (10 U/μL)
The SalI restriction enzyme recognizes G^TCGAC sites and cuts best at 37°C in O buffer.
| Concentration | 10 U/μL |
|---|