Learn More
Invitrogen™ Galacto-Light Plus™ β-Galactosidase Reporter Gene Assay System
Description
Includes
Lysis solution, substrate, reaction buffer and accelerator
- Wide dynamic range of β-galactosidase assay lets the user measure enzyme level accurately from femtogram to nanogram range
- Assay sensitivity is 100- to 1000-fold better than with either isotopic/non-isotopic assays for chloramphenicol acetyl transferase (CAT) or colorimetric/fluorescent assays for β-galactosidase, providing greater sensitivity than competing assays
- Highly sensitive assay with wide dynamic range permits detection of high and low levels of reporter without performing numerous sample dilutions
- Non-radioactive reporter gene assay kit eliminates concerns over use of radioisotopes
- Assay can be completed in about one hour, providing fast assay turnaround
Sensitive
This chemiluminescent assay exhibits over three orders of magnitude greater sensitivity compared to colorimetric β-galactosidase assays and can be completed in a fraction of time required for assays for chloramphenicol acetyltransferase (CAT) and ELISA-based reporter assays.
Ready-to-Use
The Galacto-Light Plus system incorporates chemiluminescent substrates, reaction buffer, and light-emission accelerator containing luminescence enhancers, in a ready-to-use format. The reaction buffer is supplied at a pH that aids in discrimination of endogenous β-galactosidase, while having little effect on the bacterial form of the enzyme. This enables sensitive detection even in cell lines with relatively high levels of endogenous β-galactosidase activity.
Specifications
- Assay Type: β-Gal (lacZ) Assay
- Compatible Cells: Mammalian Cells
- Detection Method: Chemiluminescent
- For Use With Equipment: Luminometer (Microplate)
- Form: Liquid
- Format: 96-well plate
- High Throughput Compatibility: High Throughput-Compatible
- Readout: End Point
- Sample Type (Specific): Cell Cultures
- Substrate: Galacton-Plus
- Substrate Properties: Chemical Substrate
- Substrate Type: Beta-Gal Substrate
- Target Enzyme: Beta-Galactosidase
Spezifikation
Spezifikation
| Product Type | β-Galactosidase Reporter Gene Assay System |
| Detection Method | Chemiluminescence |
| For Use With (Equipment) | Luminometer (Microplate) |
| Assay | β-Gal (lacZ) Assay |
| Content And Storage | This kit contains the lysis solution, substrate, reaction buffer, and accelerator. Store at 4°C. |
| Compatible Cells | Mammalian Cells |
| High-throughput Compatibility | High-throughput Compatible |
| Label or Dye | Unconjugated |
| Readout | End Point |
| Sample Type | Cell Cultures |
| Mehr anzeigen |
For Research Use Only. Not for use in diagnostic procedures.
Bitte geben Sie uns Ihr Feedback zu den Produktinhalten, indem Sie das folgende Formular ausfüllen.