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Gibco™ RPMI 1640 Medium, HEPES

Product Code. 10379144 Shop All Gibco Products
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Product Code. 10379144 Supplier Gibco™ Supplier No. 22400089
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Supplementation

RPMI 1640 Medium was originally developed to culture human leukemic cells in suspension and as a monolayer.

RPMI 1640 Medium was originally developed to culture human leukemic cells in suspension and as a monolayer. Roswell Park Memorial Institute (RPMI) 1640 Medium has since been found suitable for a variety of mammalian cells, including HeLa, Jurkat, MCF-7, PC12, PBMC, astrocytes, and carcinomas. We offer a variety of RPMI 1640 Medium modifications for a range of cell culture applications. Find the right formulation using the media selector tool.

This RPMI is modified as follows:

With: L-glutamine, HEPES, Phenol Red

The complete formulation is available.

Using RPMI

RPMI 1640 Medium is unique from other media because it contains the reducing agent glutathione and high concentrations of vitamins. RPMI 1640 Medium contains biotin, vitamin B12, and PABA, which are not found in Eagle's Minimal Essential Medium or Dulbecco's Modified Eagle Medium. In addition, the vitamins inositol and choline are present in very high concentrations. RPMI 1640 Medium contains no proteins, lipids, or growth factors. Therefore, RPMI 1640 Medium requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). RPMI 1640 Medium uses a sodium bicarbonate buffer system (2.0 g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH.

Ordreinfo

Shipping Condition: Room Temperature

TRUSTED_SUSTAINABILITY

Tekniske data

Cell Line HeLa, Jurkat, MCF-7, PC-12, PBMC, astrocytes, and carcinomas
Cell Type Leukemic Cells
Classification Animal Origin-free
Concentration 1 X
Form Liquid
Product Type RPMI 1640 Medium (Roswell Park Memorial Institute 1640 Medium)
Serum Level Standard Serum Supplementation
Sterility Sterile-filtered
Sterilization Method Sterile-filtered
With Additives Glutamine, HEPES, Phenol Red
Without Additives No Sodium Pyruvate
Manufacturing Quality cGMP-compliant under the ISO 13485 standard
Product Line Gibco
Quantity 500 mL
Shipping Condition Room Temperature
Content And Storage Storage conditions: 2-8° C. Protect from light
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture
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For Research Use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals.

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