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  7. Gibco™ Sf-900™ II SFM 

Gibco™ Sf-900™ II SFM

Product Code. 10679234
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Product Code. 10679234 Supplier Gibco™ Supplier No. 10902179

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Description

Optimized for large-scale production of recombinant proteins expressed using the baculovirus expression vector system (BEVS)

Sf-900 II SFM is a serum-free, protein-free insect cell culture medium optimized for the growth and maintenance of Spodoptera frugiperda (Sf9 and Sf21) cells. This medium is suitable for suspension and monolayer culture methods and supports growth of other lepidopteran cell lines.

  • Superior long-term, high-density growth
  • Optimized for recombinant protein production
  • Serum-free, protein-free, ready-to-use formulation
  • Scalable in bioreactors

Bioproduction, Cell Culture, Insect Cell Culture, Sf9 and Sf21 Cell Culture, Viral Vaccine Production

Order Info

Shipping Condition: Room temperature

TRUSTED_SUSTAINABILITY

Specifications

Cell Line Sf21, Sf9
Cell Type Insect Cell
Classification Protein-free, Serum-free
Form Liquid
Product Type Insect Cell Serum Free Medium (SFM)
Serum Level Serum-free
With Additives Glutamine
Product Line Gibco, Sf-900, SynerGy
Quantity 10 L
Shipping Condition Room Temperature
Species S. frugiperda, Spodoptera frugiperda
Content And Storage Storage conditions: 2°C to 8°C. Protect from light
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture
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Frequently Asked Questions (FAQs)

Can Sf9 cells in Sf-900 III SFM (Cat. No. 12659017) be thawed and grown in Sf-900 II SFM instead?

It should be okay to thaw the cells into Sf-900 II SFM. This is a richer media compared to the Sf-900 III SFM so the cells would have an easy time adapting. We would recommend taking the cells through 3 passages in the new medium before using them for any experiments as that they have enough time to adapt.

What antimicrobials can be used in insect culture and at what concentration?

Many antibiotics are suitable for use with insect cells. The following antibiotics are commonly used:
- Penicillin/Streptomycine: 50-100 U/mL; 50-100 µg/mL
- Amphotericin B (Fungizone antimycotic): 0.25 µg/mL
- Gentamicin: 0.5 mL of 10 mg/mL solution in 500 mL media (final concentration: 10 µg/mL)

Is it necessary to heat-inactivate my serum before adding it to my medium?

Heat inactivation is not necessary. Our team has routinely used serum that has not been heat-inactivated, and we have not observed any effect on cell growth or morphology.

Many cells do not require heat-inactivated FBS. Some cells prefer heat-inactivated FBS. For instance, we use heat-inactivated FBS for our insect cell lines, i.e., Sf9 and Sf21 cells.

Do you offer serum-free insect media?

Yes, we offer a variety of serum-free insect media. Please go here (http://www.thermofisher.com/us/en/home/life-science/cell-culture/insect-cell-culture/insect-cell-culture-misc/serum-free-media.html?icid=cvc-insect-media-c2t2) to view the media we offer and the differences between them.
Will your company continue to support other films?

Yes. Although we feel that the PE film exceeds many of the technical properties of the other available films, we will continue to offer and support our other currently available films through custom orders.

In what size package will my Gibco Cell Culture Bags ship?

Each bag size will arrive in a specific heavyweight corrugated box designed for shipment:
5 L: 12” x 14.25” x 5.5”
10 L: 13” x 23.5” x 6.125”
20 L: 15” x 24” x 6.125”

What are the benefits of the Gibco PE film?

The commercially available film is a very clear, multi-layered, single web film with a polyethylene contact surface that can be made into a wide range of bag sizes (250 mL to 1,000L). In addition, the film is a market leader with exceptional gas barrier properties and very low extractable and leachable results. Click here to read more about the PE Film specifications (https://www.thermofisher.com/us/en/home/life-science/cell-culture/mammalian-cell-culture/classical-media/gibco-cell-culture-bags.html).
What is the Gibco PE Film?

Based on feedback from customers, we designed a media bag portfolio that uses a consistent film for all bag sizes. This film, called PE film, is superior in function to other commercially available films. Please go here to read more about the PE Film specifications (https://www.thermofisher.com/us/en/home/life-science/cell-culture/mammalian-cell-culture/classical-media/gibco-cell-culture-bags.html).
What are Gibco Cell Culture Bags?

Gibco Cell Culture Bags are convenient, pre-filled bags in a variety of formulations and sizes, specifically designed to increase productivity and reduce costs. The bags can be formatted up to 1,000 L. Read more about these bags here (https://www.thermofisher.com/us/en/home/life-science/cell-culture/mammalian-cell-culture/classical-media/gibco-cell-culture-bags.html).

For Research Use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals.

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