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Applied Biosystems™ High-Capacity RNA-to-cDNA™ Kit

Product Code. 16271891 Shop All Applied Biosystems Products
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To receive the discount customers must purchase three of the same product at list price in a single order to receive 33% discount. There is no limit to the multiples of 3 that customers can buy. Use promo code ”27970” to get your promotional price
Product Code. 16271891 Supplier Applied Biosystems™ Supplier No. 4388950
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1973.33 EUR valid until 2026-09-25
BEST PRICE on Promo! Use promo code "27970" to get your promotional price.
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This item is not returnable. View return policy
To receive the discount customers must purchase three of the same product at list price in a single order to receive 33% discount. There is no limit to the multiples of 3 that customers can buy. Use promo code ”27970” to get your promotional price

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1 x 500 uL enzyme mix (MuLV and RNase inhibitor protein) (20X), 5 x 1 mL RT Buffer Mix (2X) (includes dNTPs, random octamers, and oligo dT-16)

The High Capacity RNA-to-cDNA Kit is a streamlined reverse transcription kit designed for optimum performance with TaqMan Gene Expression Master Mix, Power SYBR Green PCR Master Mix, and other PCR enzymes.

The High Capacity RNA-to-cDNA Kit is a streamlined reverse transcription kit designed for optimum performance with TaqMan Gene Expression Master Mix, Power SYBR Green PCR Master Mix, and other PCR enzymes. The components of the two-tube kit work together to provide sensitive and specific reverse transcription across a broad range of template amounts.

Features of the High Capacity RNA-to-cDNA Kit include:
• Fast reaction time (typically 30–60 min)
• Convenient and simple workflow with few pipetting steps
• Reliable reverse transcription of both abundant and limited targets
• Optimized two-step protocol enables multiple PCR reactions from a single RT reaction

Streamline cDNA synthesis while maintaining detection sensitivity
Amplification of a dilution series of the RNA concentration reference standard demonstrates exceptional reverse transcription and PCR efficiency across 11 orders of magnitude. This wide dynamic range allows one set of reaction conditions to detect transcripts from highly active as well as weakly expressed genes. The two-tube formulation reduces reaction time and requires fewer pipetting steps than with the High Capacity cDNA Reverse Transcription Kit, while maintaining high performance.

TRUSTED_SUSTAINABILITY

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Content And Storage

• 20X Enzyme mix (1 x 500 μL)
• 2X RT Buffer Mix (5 x 1 mL)

Store at –15 to –25°C.
2X RT Buffer Mix can be stored at 2–8°C for up to 6 months.
Format Kit
Reaction Speed 30 to 60 min.
Technique Reverse Transcription
Optimal Reaction Temperature 37°C
Quantity 500 reactions
Reverse Transcriptase MultiScribe
Ribonuclease H Activity Yes
Shipping Condition Dry Ice
For Use With (Application) Real Time PCR (qPCR), RT-PCR
Final Product Type First-Strand cDNA
No. of Reactions 500 Reactions
Reaction Format Premixed Components
Reagent Type Reverse Transcription
Size (Final Product) Up to 7 kb
Starting Material RNA
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For Research Use Only. Not for use in diagnostic procedures.

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