missing translation for 'onlineSavingsMsg'
Få mere at vide
  1. Home
  2. Products
  3. Protein Analysis Reagents
  4. Protein Purification
  5. Protein Purification Reagents and Kits
  6. Thermo Scientific™ HisPur™ Ni-NTA Superflow Agarose

Thermo Scientific™ HisPur™ Ni-NTA Superflow Agarose

Artikelnummer. 13414199 Shop alle Thermo Scientific produkter
Skift visning
Klik for at se tilgængelige muligheder
Quantity:
10 mL
50 mL
250 mL
1 L
Pakningsstørrelse:
1 L
10mL
250mL
50mL
4 product options available for selection
Product selection table with 4 available options. Use arrow keys to navigate and Enter or Space to select.
Artikelnummer. Quantity unitSize
13414199 1 L 1 L
13484189 10 mL 10mL
13404199 50 mL 50mL
13494189 250 mL 250mL
Use arrow keys to navigate between rows. Press Enter or Space to select a product option. 4 options available.
4 options
Denne vare kan ikke returneres. Se returpolicy
Artikelnummer. 13414199 Leverandør Thermo Scientific™ Leverandørnr. 25217
Discount Lab Supplies
Use promo code 28170
Se alle kampagner
 Restricted Item
Estimated Shipment: 10-08-2026
Læg i indkøbskurv
9756.50 EUR valid until 2026-12-31
BEST PRICE on Promo! Use promo code "28170" to get your promotional price.
Læg i indkøbskurv
Denne vare kan ikke returneres. Se returpolicy

Beskrivelse

Perform FPLC purification of poly-histidine-tagged proteins with this nitrilotriacetic acid (NTA) modified Superflow 6 support charged with divalent nickel (Ni+2).

Perform FPLC purification of poly-histidine-tagged proteins with Thermo Scientific™ HisPur Ni-NTA Superflow Agarose, a nitrilotriacetic acid (NTA) modified Superflow 6 support charged with divalent nickel (Ni+2).

Additional Information and Online Ordering

HisPur Ni-NTA Superflow Agarose is a highly crosslinked, durable resin that does not compress at flow rates used for medium- or large-scale FPLC purifications. The resin holds up well to a variety of chemicals and pH values, and is compatible with common clean-in-place procedures. Compared to cobalt and other ligands used for immobilized metal affinity chromatography (IMAC), nickel provides greater capacity for His-tagged protein purification. HisPur Ni-NTA Superflow Agarose exhibits a high dynamic binding capacity across a range of flow rates, making it an excellent choice for larger scale purifications.

Highlights:

  • High capacity – binds greater than 20mg of His-tagged GFP per mL of resin at a linear flow rate of 300cm/hour

  • High purity – provides greater than 80% purity of eluted fractions when used to purify from lysates

  • Versatile – can be used to purify proteins under native and denaturing conditions

  • Robust – highly crosslinked beads tolerate linear flow rates up to 1200cm/hour

  • Compatible – maintains function in a wide variety of chemicals and pH conditions

  • Cost effective – competitively priced resin is stable through multiple cycles of cleaning and reuse

Recommended for:

Medium- to large-scale FPLC purification of polyhistidine tagged proteins

TRUSTED_SUSTAINABILITY

Tekniske data

Capacity (Metric) 20 mg His-Tagged GFP Binding per mL Resin
Content And Storage Store at 4°C
Form Liquid Suspension
Product Line HisPur
Quantity 1 L
Target His-Tagged Protein
Type Agarose
Stationary Phase Divalent Nickel, Metal Chelate
Format Bottle
Column Type Affinity, Agarose Resin

For Research Use Only. Not for use in diagnostic procedures.

Produkttitel
Vælg et problem

Ved at klikke på Send, anerkender du, at du kan blive kontaktet af Fisher Scientific med hensyn til den feedback, du har givet i denne formular. Vi deler ikke dine oplysninger til andre formål. Alle angivne kontaktoplysninger skal også vedligeholdes i overensstemmelse med vores Privatlivspolitik.