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Description
Papain cleaves peptide bonds of basic amino acids, leucine, or glycine. It is also hydrolyzes esters and amides.
- CAS Number: 9001-73-4
- EC Number: 232-627-2
- MDL Number: MFCD00131791
- Format: Milky white solution
- Specific Activity: ≤10 units/mg Protein
- Extinction coefficient: 25.0
- Isoelectric point (pI): pH 9.6
- Optimum pH: 6.0 to 7.0
Used to produce Fab fragments of antibodies, cell dissociation, morphological isolation of intact cortical neurons from postnatal rats. Limited papaindigestion is used for structural studies of enzymes and other proteins. In addition, papain is used in red cell serology to modify red cell surface to enhance or destroy reactivity of many red cell antigens. Useful for platelet serology studies and has also been used in enzymatic synthesis of amino acids, peptides, and other molecules.
Specifications
Specifications
| Concentration | 2X |
| Color | Milky White |
| pH | 6 to 7 |
| For Use With (Application) | Produce Fab fragments of Antibodies, cell dissociation, morphological isolation of intact cortical neurons from Postnatal Rats |
| Content And Storage | 4°C |
| Source | Papaya Latex |
| Quantity | 500 mg |
| Product Type | Papain |
| Molecular Weight (g/mol) | 23000 |
| Inhibitors | Sulfhydryl groups including heavy Metals, Carbonyl reagents, Aldehydes, Benzoylamidoacetonitrile, AEBSF, antipain, cystatin, E-64, leupeptin, PMSF, TLCK and TPCK |
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Safety and Handling
- Papain
- Danger
- Serious eye damage/eye irritation Category 2
- Respiratory sensitiser Category 1
- Skin corrosion/irritation Category 2
- Specific target organ toxicity Category 3
- H315-Causes skin irritation.
- H319-Causes serious eye irritation.
- H334-May cause allergy or asthma symptoms or breathing difficulties if inhaled.
- H335-May cause respiratory irritation.
- P101-If medical advice is needed, have product container or label at hand.
- P102-Keep out of reach of children.
- P103-Read label before use.
- P261-Avoid breathing dust/fume/gas/mist/vapours/spray.
- P284-[In case of inadequate ventilation] wear respiratory protection.
- P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
- P321-Specific treatment (see on this label).
- P405-Store locked up.
- P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.
- MIXTURE LIST-Contain: papain
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