missing translation for 'onlineSavingsMsg'
Learn More
  1. Home
  2. Products
  3. Immunoassay Reagents and Kits
  4. Life Science Research ELISA Kits
  5. Invitrogen™ Human TNF alpha ELISA Kit 

Invitrogen™ Human TNF alpha ELISA Kit

Product Code. 10737663
Change view
Click to view available options
Quantity:
2 x 96 Tests
5 x 96 Tests
96 Tests
Conditionnement:
192 tests
96 tests
Pack of 5
3 product options available for selection
Product selection table with 3 available options. Use arrow keys to navigate and Enter or Space to select.
Code produit Quantity unitSize
10737663 96 Tests 96 tests
10008932 2 x 96 Tests 192 tests
10632644 5 x 96 Tests Pack of 5
Use arrow keys to navigate between rows. Press Enter or Space to select a product option. 3 options available.
3 options
Les retours ne sont pas autorisés pour ce produit. Afficher la politique du retour.
Code produit 10737663 Fournisseur Invitrogen™ Code fournisseur KHC3011

Please to purchase this item. Need a web account? Register with us today!

714.40 EUR valid until 2026-06-30
BEST PRICE on Promo! Use promo code "28170" to get your promotional price.
Les retours ne sont pas autorisés pour ce produit. Afficher la politique du retour.

Description

Includes

Human TNF-α Antibody Coated 96-Well Plate
Human TNF-α Standard
Standard Diluent Buffer
Human TNF-α Detection Antibody
Streptavidin-HRP
HRP Diluent
Wash Buffer Concentrate (25X)
Stabilized Chromogen, TMB
Stop Solution
Plate Covers
etailed protocol with validation tests

ELISA

The Human Tumor Necrosis Factor alpha (Hu TNFα) ELISA quantitates Hu TNFα in human serum, plasma, buffered solution, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu TNFα. Principle of the method The Human TNFα solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

TNF alpha is a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophage and bind to its receptors, TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. TNF alpha is involved in the regulation of immune cells, cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. TNF alpha exists as a multimer of two, three, or five noncovalently linked units, but shows a single 17 kDa band following SDS-PAGE under non-reducing conditions. Knockout studies in mice also suggested the neuroprotective function of TNF alpha, and has been observed to causes tumor necrosis when injected into tumor-bearing mice. Other functions of TNF-alpha include its role in the immune response to bacterial, viral, parasitic and certain fungal infections, as well as its role in the necrosis of specific tumors. TNF alpha causes cytolysis or cytostasis of certain transformed cells, being synergistic with interferon-gamma in its cytotoxicity. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer.

Informations sur la commande

Shipping Condition: Dry Ice

TRUSTED_SUSTAINABILITY

Spécification

Accession Number P01375
Assay Range 15.6 to 1000 pg/mL
Assay Sensitivity 1.7 pg/mL
Conjugate HRP
Product Type ELISA
Sample Type Plasma, Serum, Supernatant
Target Species Human
For Use With (Application) Colorimetric Detection
For Use With (Equipment) Colorimetric Microplate Reader
Gene ID (Entrez) 7124
Gene Symbol DIF-alpha,TNFA,TNFSF2,TNLG1F,TNF
Incubation Time 4 hr.
Interassay CV 7.5%
Intraassay CV 4.4
Kit Contents Pre-coated 96 well plate, Standard, Standard Dilution Buffer, Biotinylated Detection Antibody, Streptavidin-HRP, HRP Diluent, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
Label or Dye HRP
Marker Gene TNF-α
Quantity 96 Tests
Regulatory Status RUO
Sample Volume Plasma 100 μL, Serum 100 μL, Supernatant 100 μL
Storage Requirements 2°C to 8°C
Target TNF alpha
Test Time 1 hrs 20 mins
Total Assay Time 4 hrs
Afficher plus Afficher moins

For Research Use Only. Not for use in diagnostic procedures.

Nom du produit
Sélectionnez un problème

En cliquant sur Soumettre, vous reconnaissez que vous pouvez être contacté par Fisher Scientific au sujet des informations que vous avez fournies dans ce formulaire. Nous ne partagerons pas vos informations à d'autres fins. Toutes les informations de contact fournies seront également conservées conformément à notre politique de confidentialité. Politique de confidentialité.