PeproTech PlGF-1 Polyclonal Antibody, PeproTech®, Invitrogen™

Description

AA Sequence of recombinant protein: MLPAVPPQQW ALSAGNGSSE VEVVPFQEVW GRSYCRALER LVDVVSEYPS EVEHMFSPSC VSLLRCTGCC GDENLHCVPV ETANVTMQLL KIRSGDRPSY VELTFSQHVR CECRPLREKM KPERCGDAVP RR. Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human PlGF-1. Anti-Human PlGF-1-specific antibody was purified by affinity chromatography employing an immobilized Human PlGF-1 matrix. Sandwich ELISA: To detect Human PlGF-1 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human PlGF-1 (500-P226Bt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human PlGF-1. Western Blot: To detect Human PlGF-1 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human PlGF-1 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

PlGF-1 is an angiogenic factor that belongs to the cysteine-knot superfamily of growth factors. PlGF-1 is expressed in placental tissues, the colon, and mammary carcinomas. It signals through the VEGFR-1/FLT1 receptor, and stimulates endothelial cell proliferation and migration.