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Invitrogen™ PureLink™ Microbiome DNA Purification Kit
Description
The Invitrogen™ PureLink™ Microbiome DNA Purification Kit uses proven PureLink™ spin column technology for robust yields of purified DNA ready for downstream PCR, sequencing or other applications. The highly efficient triple lysis approach, fast removal of inhibitors, and versatility makes PureLink the ultimate kit for microbiome research projects as well as programs aimed at rapid detection of pathogenic bacteria in various samples.
- Efficient lysis of all microorganisms (including durable species with thicker and more complex cell walls) by a combination of heat, chemical, and mechanical disruption with specialized beads
- Elimination of inhibitory compounds by precipitation using a novel cleanup buffer
- Streamlined protocols for numerous biological samples
- Recovery of highly pure DNA compatible with common downstream applications such as qPCR and next-generation sequencing
Certifications
Certificates of Analysis for each lot
Warranty
Product performance guaranteed for 1 year from date of sale
Specifications
Specifications
| Column Type | Spin Column |
| Content And Storage | • 40 mL S1 Lysis Buffer; room temperature • 5 mL S2 Lysis Enhancer; room temperature • 12.5 mL S3 Cleanup Buffer; room temperature • 45 mL S4 Binding Buffer; room temperature • 13 mL S5 Wash Buffer (concentrated); room temperature • 5 mL S6 Elution Buffer; room temperature • 50 PureLink Columns with Collection Tubes; room temperature • 100 PureLink Collection Tubes; room temperature • 50 Bead Tubes; room temperature |
| Isolation Technology | Silica Spin Column |
| Scale | Mini |
| Sample Type | Microbial Culture, Saliva, Soil, Stool, Host DNA from Stool, Swabs (Buccal, Vaginal, Skin, Rectal, or Environmental), Cells, Food and Environmental |
| Elution Volume | 50 to 200 μL |
| Final Product Type | Microbial and host DNA |
| For Use With (Application) | PCR, Southern blotting, sequencing, nucleic acid labeling, hybridization |
| High-throughput Compatibility | Not High-throughput Compatible (Manual) |
| Quantity | 50 Preps kit |
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Safety and Handling
- PureLink Microbiome DNA Purification Kit
- Warning
- Acute toxicity Category 4
- Serious eye damage/eye irritation Category 2
- Skin corrosion/irritation Category 2
- H302-Harmful if swallowed.
- H315-Causes skin irritation.
- H319-Causes serious eye irritation.
- H332-Harmful if inhaled.
- P261-Avoid breathing dust/fume/gas/mist/vapours/spray.
- P264-Wash hands thoroughly after handling.
- P270-Do not eat, drink or smoke when using this product.
- P271-Use only outdoors or in a well-ventilated area.
- P280-Wear protective gloves/protective clothing/eye protection/face protection.
- P301+P310-IF SWALLOWED: Immediately call a POISON CENTER/doctor /
- P302+P352-IF ON SKIN: Wash with plenty of water/soap.
- P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.
- P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
- P312-Call a POISON CENTER/doctor/if you feel unwell.
- P330-Rinse mouth.
- P332+P313-If skin irritation occurs: Get medical advice/attention.
- P337+P313-If eye irritation persists: Get medical advice/attention.
- P362+P364-Take off contaminated clothing and wash it before reuse.
- P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.
- MIXTURE LIST-Contains : Guanidine hydrochloride, Thiocyanic acid
For research use only. Not for use in diagnostic procedures.
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