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  7. Applied Biosystems™ TaqMan™ Gene Expression Assay, VIC primer-limited

Applied Biosystems™ TaqMan™ Gene Expression Assay, VIC primer-limited
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Artikelnummer. 11917021 Shop alle Applied Biosystems produkter
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Quantity:
L (2900 reactions), made to order
M (750 reactions), made to order
S (360 reactions), made to order
Pakningsstørrelse:
Each
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Artikelnummer. Quantity unitSize
11917021 S (360 reactions), made to order Each
11907021 M (750 reactions), made to order Each
11937021 L (2900 reactions), made to order Each
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This Product Requires Additional Configuration

Please order Applied Biosystems™ TaqMan™ Gene Expression Assays through the Gene Expression Search Tool.
Artikelnummer. 11917021 Leverandør Applied Biosystems™ Leverandørnr. 4448484

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286.09 EUR valid until 2026-06-30
BEST PRICE on Promo! Use promo code "27930" to get your promotional price.
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This Product Requires Additional Configuration

Please order Applied Biosystems™ TaqMan™ Gene Expression Assays through the Gene Expression Search Tool.

Beskrivelse

Applied Biosystems TaqMan Gene Expression Assays, VIC primer-limited, are used for quantitative real-time PCR analysis of gene expression and consist of a pair of unlabeled PCR primers and a TaqMan probe with a dye label (VIC) on the 5' end and a minor groove binder (MGB) and nonfluorescent quencher (NFQ) on the 3' end.

Applied Biosystems TaqMan Gene Expression Assays, VIC primer-limited, are used for quantitative real-time PCR analysis of gene expression and consist of a pair of unlabeled PCR primers and a TaqMan probe with a dye label (VIC) on the 5' end and a minor groove binder (MGB) and nonfluorescent quencher (NFQ) on the 3' end. These assays are primer-limited and ideal for multiplex reactions looking at two different gene targets in the same qPCR well. Note that for digital PCR, primer-limiting is typically not recommended. The full-concentration TaqMan assay (Cat. No. 4448491) is suggested if you are using dPCR.

Features include:

  • Easy to use—just add cDNA and master mix and run the qPCR—no melt curves required
  • Specific—TaqMan assays use proprietary MGB-containing probes that can be up to 15 bases shorter than non-MGB probes, improving the specificity of the assay
  • Sensitive—TaqMan assays are ideal for measuring low levels of expression or low-abundance targets
  • Accurate—identify small fold-changes with high accuracy of quantitation
  • Extensive content—over 1.8 million predesigned assays available for over 25 different species
  • Gold-standard TaqMan qPCR chemistry—TaqMan assays draw on Thermo Fisher Scientific's bioinformatics assay design pipeline to help ensure high specificity and minimal cross-reactivity, even for gene variants with high sequence homology
  • Ideal for multiplexing—combine one FAM-labeled and one VIC-labeled assay to create a duplex assay for two different gene targets in the same qPCR well
  • Ideal for control assays—use a VIC primer-limited assay for high-expressing endogenous control genes

Approximate ship time

Made to order: 4–6 days in North America, 6–10 days in Europe

Pre-formulated assay

  • 2 unlabeled PCR primers (150 nM each final 1X concentration)
  • 1 VIC dye-labeled TaqMan MGB probe (250 nM final 1X concentration)

TaqMan Gene Expression assays are the gold standard in real-time PCR gene expression studies, built on more than 20 years of experience. Each assay includes target primers and a sequence-specific probe optimized for the best functional performance. No additional design, optimization, or melt curve analysis is needed. Available in a wide variety of formats and species, new assay designs are constantly added to help meet your research needs. TaqMan assays have been cited in over 40,000 publications and are backed by more than 350 patents. All of our predesigned TaqMan Gene Expression assays are covered by the TaqMan Assays Guarantee.*

Recommended master mix: TaqMan Fast Advanced Master Mix (Cat. No. 4444556)

*Subject to terms and conditions. For complete details, go to www.thermofisher.com/taqmanguarantee.

TRUSTED_SUSTAINABILITY

Tekniske data

Concentration 20X
Content And Storage 1 tube containing a 20X (S and M sizes) or 60X (L size) mix of pre-formulated assay (1 probe and 2 primers).

Store at -15 to -25°C.
Detection Method Primer-probe
Format Tube
GC-Rich PCR Performance High
PCR Method qPCR
Reaction Speed Fast
For Use With (Equipment) 7500 System, 7500 Fast System, 7900HT System, StepOne™, StepOnePlus™, ViiA™ 7 System, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 7 Flex, QuantStudio 6 Pro, QuantStudio 7 Pro, QuantStudio™ 12k Flex
Product Line TaqMan
Product Type Gene Expression Assay
Quantity S (360 reactions), made to order
Shipping Condition Room Temperature
Fidelity (vs. Taq) 60 X
No. of Reactions 360 reactions
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For Research Use Only. Not for use in diagnostic procedures.

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