Applied Biosystems™ TaqMan™ Protein Assays Core Reagents Kit with MasterMix

Contains both the Core Reagents Base Kit and the TaqMan Protein Assays Fast Master Mix Kit.

The Core Reagents Base Kit contains the reagents required to perform the ligation and PCR amplification steps of TaqMan™ protein expression experiments. The Universal PCR Assay contains optimized concentrations of TaqMan™ probe and oligonucleotide primers (forward and reverse) that are designed to amplify a region of the protease-treated ligation product for detection on an Applied Biosystems real-time PCR system. The enzyme concentration, ionic strength, and the pH of the TaqMan™ Protein Expression Fast Master Mix are optimized for the amplification of ligated oligonucleotide target sequences prepared using TaqMan™ Protein Expression Assay reagents. The DNA polymerase included in the master mix is designed for maximum PCR efficiency and specificity when used with the ligation chemistry.