Real Time PCR Reagents and Kits
Real Time PCR Reagents and Kits
Products designed for use during real-time (quantitative) PCR procedures. Includes master mixes, DNA polymerases, reverse transcriptases, size standards, kits, and reagents.
Reverse transcription polymerase chain reaction (RT-PCR) is a laboratory technique that combines the reverse transcription of RNA into DNA and the subsequent amplification of the DNA using PCR.
In RT-PCR, a reverse transcriptase enzyme converts an RNA template to its complementary DNA (cDNA). The cDNA then becomes the template for PCR amplification.
A typical RT-PCR kit includes the main components needed for the assay:
- Buffer
- Primers – a combination of deoxynucleotide triphosphates (dNTPs) of the four bases of adenine (dATP), cytosine (dCTP), guanine (dGTP), and thymine (dTTP)
- Reverse transcriptase reagent
The combination of RT-PCR and qPCR techniques (sometimes labelled RT-qPCR) is often used in research and clinical labs to analyze gene expression and quantify viral RNA. Since this method uses fluorescence to track the amplification reaction, these kits may also include:
- Fluorogenic primers or labeled fluorogenic probes
- Fluorescent dye
Reagents for cDNA PCR can be purchased separately or as cDNA synthesis kits.
RT-PCR is commonly used for RNA detection, molecular cloning, and gene sequencing. RT-qPCR is used for gene expression analysis, RNAi or microarray validation, pathogen detection, genetic testing, and other disease research.
Applied Biosystems™ TaqPath™ qPCR Master Mix, CG
Designed and all lots are functionally tested to help ensure lot-to-lot reproducibility for Ct consistency and dynamic range across a wide variety of assays.
Promotions are available
Invitrogen™ SuperScript™ III Platinum™ One-Step qRT-PCR Kit
Combine the most powerful reverse transcriptase and DNA polymerase technologies to deliver precise and accurate analysis of gene expression in a convenient one-step format.
Promotions are available
Thermo Scientific™ RapidFinder™ Halal ID Kit for RT-PCR
Determine the Halal status of food and feeds using this simple, reliable, sensitive PCR detection method for pork DNA
TaqMan™ TaqMan™ MicroRNA Reverse Transcription Kit
Provides the necessary components for 200 reactions of optimal performance in TaqMan™ MicroRNA Assays.
Thermo Scientific™ RapidFinder™ Pork ID Kit
The RapidFinder™ Pork ID Kit enables the simple and reliable detection of pork in food and feed samples.
Invitrogen™ ROX Reference Dye
ROX Reference Dye is designed to normalize the fluorescent reporter signal in real-time quantitative PCR or RT-PCR.
Promotions are available
Thermo Scientific™ SureTect™ Escherichia coli O157:H7 and STEC Screening PCR Assay
Rapidly detect E. coli O157:H7, stx, eae, in food samples in as little as 10 hours with highly-specific, simple to use real-time PCR ThermoScientific Suretect Escherichia coli O157:H7 and STEC Screening PCR Assay.
Invitrogen™ EXPRESS One-Step SYBR™ GreenER™ Kit, with premixed ROX
Promotions are available
Thermo Scientific™ Verso 1-step RT-qPCR Kit, SYBR Green, with separate ROX vial
The Verso RT enzyme delivers high processivity and can be used at temperatures up to 57°C, delivering effective transcription through difficult RNA secondary structures.
Promotions are available
SuperScript™, Platinum™, SYBR™ SuperScript™ III Platinum™ SYBR™ Green One-Step qRT-PCR Kit
Combine the most powerful reverse transcriptase and DNA polymerase technologies to deliver precise and accurate analysis of gene expression in a convenient one-step format.
Promotions are available
Applied Biosystems™ Absolute Q™ dPCR SARS-CoV-2 Wastewater Surveillance Kit
The Applied Biosystems™ Absolute Q™ dPCR SARS-CoV-2 Wastewater Surveillance Kit enables fast, sensitive, and absolute quantification of SARS-CoV-2 from isolated wastewater nucleic acid using the QuantStudio™ Absolute Q™ Digital PCR System.
Thermo Scientific™ Verso 1-step RT-qPCR Kit, SYBR Green, ROX
The Verso RT enzyme delivers high processivity and can be used at temperatures up to 57°C, delivering effective transcription through difficult RNA secondary structures.
Promotions are available
Applied Biosystems™ TaqMan™ Protein Assays Core Reagents Kit with MasterMix
Contains both the Core Reagents Base Kit and the TaqMan Protein Assays Fast Master Mix Kit.
SureTect™ Listeria Species PCR Assay
Rapidly detect Listeria species in food and environmental samples with the Thermo Scientific™ SureTect™ Listeria Species PCR Assay.
Applied Biosystems™ TaqMan™ Brucella spp Detection Kit
TaqMan™ Brucella spp. Detection Kit