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  7. TaqMan™, RNA-to-CT™ TaqMan™ RNA-to-CT™ 1-Step Kit

TaqMan™, RNA-to-CT™ TaqMan™ RNA-to-CT™ 1-Step Kit

Product Code. 10734807 Shop All Applied Biosystems Products
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200 Reactions
2000 Reactions
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200 reactions
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Product Code. No. of Reactions unitSize
10734807 200 Reactions 200 reactions
10158014 40 Reactions Each
10045714 2000 Reactions 1 set
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Product Code. 10734807 Supplier TaqMan™, RNA-to-CT™ Supplier No. 4392938

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Description

Delivers consistent RNA target quantification for a wide variety of assays and is validated with Applied Biosystems™ comprehensive library of TaqMan™ Gene Expression assays.

The TaqMan™ RNA-to-CT™ One-Step Kit is recommended for a variety of applications including general gene expression studies, biomarker discovery, and microarray validation.

  • Accurate across a wide dynamic range for dependable performance
  • High sensitivity, enabling low copy number detection and precise quantification
  • Validated with TaqMan™ Gene Expression Assays for easy experimental setup
  • Consistent performance with a wide variety of targets, including AT-rich, GC-rich and long sequences
  • Fewer pipetting steps means less hands-on-time and less chance for errors

Efficiency across a Broad Range of Input Concentrations
  • Maximum flexibility in RNA template input quantity requires optimal real-time PCR efficiency across a broad range of template quantities
  • The result is comparable to that of the TaqMan™ RNA-to-CT™ 2-Step Kit, and is a significant improvement in dynamic range and sensitivity over the TaqMan™ One-Step RT-PCR Master Mix Reagents.

High Sensitivity at Low Target Concentration
  • The sensitivity of TaqMan™ RNA-to-CT™ One-Step Kit was validated using a synthetic RNA template for which copy number is precisely known
  • Significant sampling error occurs when measuring low quantities of target, so statistical analysis is required for proper evaluation using multiple replicates
  • Statistical analysis indicates high confidence of sample quantification based on a T-test, consistent with as few as 10 copies of target.

Order Info

Shipping Conditions: Wet Ice

TRUSTED_SUSTAINABILITY

Specifica

Content And Storage Contains sufficient reagents for 200 reactions at 50 μl reaction volumes. Includes:
• 1 × 5 ml tube of 2X master mix containing AmpliTaq Gold™ DNA Polymerase UP, dNTPs (including dUTP), Passive Reference 1, and optimized buffer components. Store at -15 to -25°C.
• 1 × 250 μl tube of 40X RT enzyme mix containing ArrayScript™ UP Reverse Transcriptase, RNase Inhibitor. Store at -15°C to -30°C.
Detection Method Primer-probe
PCR Method 1-step RT-qPCR
Polymerase AmpliTaq Gold DNA Polymerase
Reaction Speed Standard
For Use With (Equipment) 7000 System, 7300 System, 7500 System, 7700 System, 7900HT System,Applied Biosystems StepOnePlus™ Fast Real-Time PCR System, StepOne™, Standard Mode, StepOnePlus™, Standard Mode
Passive Reference Dye ROX (Pre-mixed)
Product Line RNA-to-CT, TaqMan
Product Type One-Step RT-PCR Kit
Purity or Quality Grade UP (Ultra Pure)
Quantity 200 reactions
Reverse Transcriptase ArrayScript™ UP
Shipping Condition Wet Ice
Sufficient For 200 Reactions
For Use With (Application) Gene Expression
No. of Reactions 200 Reactions
Sample Type RNA
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For Research Use Only. Not for use in diagnostic procedures.

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