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Invitrogen™ ViewRNA™ Colorimetric Tissue 2-Plex Kits
Description
ViewRNA Colorimetric Tissue assays are RNA in situ hybridization (ISH) assays for the reliable detection of mRNA targets within tissue sections at single-copy sensitivity. The ViewRNA assays use proprietary chemistry for the target-specific probe sets along with branched DNA (bDNA) technology to amplify target-specific signal and achieve superior signal-to-noise ratios compared to traditional colorimetric ISH signal amplification methods, which often amplify both the signal and background.
When combined with ViewRNA Tissue probe sets (purchased separately), the ViewRNA 2-Plex Kits enable the colorimetric detection of two mRNA targets in tissue sections simultaneously using the Fast Red, Blue, and DAB substrates. ViewRNA 1-Plex Kits for the colorimetric detection of a single RNA target are also available.
ViewRNA Tissue assays are RNA in situ hybridization (ISH) assays for the reliable detection of one or two mRNA targets within tissue sections. Visualize any gene in any tissue with single-copy sensitivity and no radioactivity. And in case your needs are not met by one of our 6500 catalog probe sets, we have decades of bioinformatics expertise and will design custom probe sets to any mRNA target of interest, free of charge.
With ViewRNA Tissue assays, you can achieve:
• Robust, single mRNA molecule detection in tissue sections
• Accuracy and specificity inherent to branched DNA amplification technologies
• Simultaneous detection of two RNA targets
The ViewRNA Tissue Assay is compatible with formalin-fixed, paraffin-embedded (FFPE) and cryopreserved tissue. Whereas traditional ISH technologies often amplify both signal and background, ViewRNA assays use branched DNA (bDNA) technology to amplify target-specific signal and achieve superior signal-to-noise ratios.
To meet various experimental needs, the ViewRNA Tissue assay is provided in a variety of kits and modules. The ViewRNA Tissue Core Kit and ViewRNA Modules can be purchased separately in different combinations to mix and match and create a colorimetric ViewRNA assay for detection of up to two RNA targets. For added convenience, ViewRNA 1-Plex and 2-Plex Kits containing the ViewRNA Tissue Core Kit and ViewRNA Modules can also be purchased, then supplemented with the ViewRNA Tissue Core Kit and ViewRNA Modules as needed for additional assays or substrates. The ViewRNA 1-Plex and 2-Plex Kits include sufficient reagents to stain 24 or 96 tissue sections.
ViewRNA Red-Blue 2-Plex Kit
The ViewRNA Red-Blue 2-Plex Kit includes the ViewRNA Tissue Core Kit, ViewRNA Fast Red Module, and ViewRNA Fast Blue Module for simultaneous detection of two mRNA targets with red and blue colored precipitate for ViewRNA colorimetric ISH tissue staining. The alkaline phosphatase (AP) substrate Fast Red is for use with ViewRNA Type 1 or Type 6 Probe Sets and the alkaline phosphatase (AP) substrate Fast Blue is for use with ViewRNA Type 6 or Type 1 Probe Sets (ViewRNA probe sets are sold separately).
ViewRNA Red-Brown 2-Plex Kit
The ViewRNA Red-Brown 2-Plex Kit includes the ViewRNA Tissue Core Kit, ViewRNA Fast Red Module, and ViewRNA DAB Module for simultaneous detection of two mRNA targets with red and brown colored staining for ViewRNA colorimetric ISH tissue staining. The alkaline phosphatase (AP) substrate Fast Red is for use with ViewRNA Type 1 or Type 6 Probe Sets and the horseradish peroxidase (HRP) substrate DAB is for use with ViewRNA Type 4 Probe Sets (ViewRNA probe sets are sold separately).
ViewRNA Blue-Brown 2-Plex Kit
The ViewRNA Blue-Brown 2-Plex Kit includes the ViewRNA Tissue Core Kit, ViewRNA Fast Blue Module, and ViewRNA DAB Module for simultaneous detection of two mRNA targets with blue and brown colored staining for ViewRNA colorimetric ISH tissue staining. The alkaline phosphatase (AP) substrate Fast Blue is for use with ViewRNA Type 6 or Type 1 Probe Sets and the horseradish peroxidase (HRP) substrate DAB is for use with ViewRNA Type 4 Probe Sets (ViewRNA probe sets are sold separately).
Specifications
Specifications
| Content And Storage | • 21 mL 100X Pretreatment Solution (2–8°C), also available separately • 12 mL Probe Set Diluent (2–8°C), also available separately • 10 mL PreAmplifier Mix (2–8°C) • 10 mL Amplifier Mix (2–8°C) • 24 mL Label Probe Diluent (2–8°C), also available separately • 180 μL Protease Solution (2–8°C, do not freeze), also available separately • 144 mL Wash Buffer Component 1 (15–30°C) • 280 mL Wash Buffer Component 2 (15–30°C) • 320 μL Fast Red Reagent 1 (2–8°C) • 320 μL Fast Red Reagent 2 (2–8°C) • 4 vials of Fast Red Reagent 3 - lyophilized (2–8°C), add 120 μL of ddH2O per vial to dissolve • 20 mL Fast Red Reaction Buffer (2–8°C) • 10 mL AP (Alkaline Phosphatase) Enhancer Solution (2–8°C), also available separately • 45 μL Label Probe Type 1 - AP (2–8°C) • 420 μL Fast Blue Reagent 1 (2–8°C) • 420 μL Fast Blue Reagent 2 (2–8°C) • 420 μL Fast Blue Reagent 3 (2–8°C) • 20 mL Fast Blue Reaction Buffer (2–8°C) • 10 mL AP Stop Solution (2–8°C) • 45 μL Label Probe Type 6 - AP (2–8°C, do not freeze) |
| Detection Method | Chromogenic, Fluorescence |
| Format | Kit |
| Final Product Type | RNA (Labeled) |
| Labeling Target | RNA (General) |
| For Use With (Application) | In Situ Hybridization (ISH) |
| Product Line | ViewRNA |
| Product Type | ViewRNA Red-Blue 2-Plex Kit |
| Quantity | 24 assays |
| Shipping Condition | Assay ships as 4 components: QVT0400A (Wet Ice), QVT0400B (Room Temperature), QVT0410 (Wet Ice), QVT0420 (Wet Ice) |
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