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Applied Biosystems™ TaqMan™ Universal PCR Master Mix, no AmpErase™ UNG

TaqMan™ Universal PCR Master Mix, No AmpErase™ UNG is the ideal reagent solution when you need a Master Mix for multiple 5' nuclease DNA applications.

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Specifications

Real Time PCR Master Mixes

Shipping Condition	Wet Ice
Polymerase	AmpliTaq Gold DNA Polymerase
GC-Rich PCR Performance	High
Product Type	Universal PCR Master Mix
Passive Reference Dye	ROX (Pre-mixed)
For Use With (Equipment)	7000 System, 7300 System, 7500 System, 7700 System, 7900HT System,Applied Biosystems StepOnePlus™ Fast Real-Time PCR System, GeneAmp 9600, StepOne™, Standard Mode, StepOnePlus™, Standard Mode
Sample Type	DNA (Genomic), cDNA
Reaction Speed	Standard
For Use With (Application)	Gene Expression,Genotyping,miRNA analysis
PCR Method	qPCR
Product Line	TaqMan™
Detection Method	Primer-probe

SYBR™ Power SYBR™ Green PCR Master Mix

Offers superior sensitivity and reproducibility, detecting as few as two copies of a target gene over a broad range of template concentrations.

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Specifications

Real Time PCR Master Mixes

GC-Rich PCR Performance	High
Purity or Quality Grade	LD (Low DNA)
Concentration	2X
For Use With (Application)	Gene Expression
PCR Method	qPCR
Detection Method	SYBR
Polymerase	AmpliTaq Gold DNA Polymerase, AmpliTaq Gold LD
Product Type	Real Time PCR SYBR Master Mix
Passive Reference Dye	ROX (Pre-mixed)
For Use With (Equipment)	7300 System, 7500 System, 7700 System, 7900HT Fast System,Applied Biosystems StepOnePlus™ Fast Real-Time PCR System, QuantStudio™ 12k Flex, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 7, StepOne™, Standard Mode, StepOnePlus™, Standard Mode, ViiA™ 7 System
Sample Type	DNA (Genomic), cDNA
Reaction Speed	Standard
Label or Dye	SYBR Green
Product Line	SYBR™

Applied Biosystems™ Human GAPD (GAPDH) Endogenous Control (FAM™/MGB probe, non-primer limited)

Human GAPD (GAPDH) Endogenous Control (FAM™/MGB probe, non-primer limited)

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Specifications

Real Time PCR Assays and Arrays

Shipping Condition	Room Temperature
Content And Storage	Control Primer-Probe Set(s)
Form	Liquid
Gene Symbol	GAPDH
Green Features	Sustainable packaging
Amplicon Size	122
Concentration	20X
For Use With (Application)	Gene Expression
PCR Method	2-step RT-qPCR
Detection Method	Primer-probe
Accession Number	NM_002046.3
Target	GAPDH
Format	Tube
Label or Dye	FAM
Species	Human
Product Line	FAM™, TaqMan™

Applied Biosystems™ TaqMan™ Universal PCR Master Mix

TaqMan Universal PCR Master Mix is the ideal reagent solution when you need a master mix for multiple 5' nuclease DNA applications.

Promotions are available

Pricing and Availability
Specifications

Real Time PCR Master Mixes

Polymerase	AmpliTaq Gold DNA Polymerase
GC-Rich PCR Performance	High
Product Type	Universal PCR Master Mix
Passive Reference Dye	ROX (Pre-mixed)
For Use With (Equipment)	7000 System, 7300 System, 7500 System, 7700 System, 7900HT System,Applied Biosystems StepOnePlus™ Fast Real-Time PCR System, GeneAmp 9600, StepOne™, Standard Mode, StepOnePlus™, Standard Mode
Sample Type	DNA (Genomic), cDNA
Reaction Speed	Standard
For Use With (Application)	Gene Expression,Genotyping,miRNA analysis
PCR Method	qPCR
Product Line	TaqMan™
Detection Method	Primer-probe

Cytiva PuReTaq Ready-To-Go™ PCR Beads

Pre-mixed, pre-dispensed reaction beads optimized for performing standard PCR amplifications. Cytiva Lifescience™ illustra™ PuReTaq Ready-To-Go™ PCR Beads are stable at ambient temperature.

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Specifications

Standard PCR Reagents and Kits

Content And Storage	Room temperature stable beads containing ∼ 2.5 units of recombinant PuReTaq DNA Polymerase, dATP, dCTP, dGTP, dTTP, stabilizers, BSA and reaction Buffer, When a bead is reconstituted to a 25μL final volume, the concentration of each dNTP
Quantity	1 Kit
For Use With (Application)	Standard PCR

Thermo Scientific™ RapidFinder™ Beef ID Kit

The RapidFinder™ Beef ID Kit enables the simple and reliable detection of beef in food and feed samples.

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Real Time PCR Reagents and Kits

Applied Biosystems™ High-Capacity cDNA Reverse Transcription Kit

The High Capacity cDNA Reverse Transcription Kit, available with or without RNase Inhibitor, contains all components necessary for the quantitative conversion of up to 2 μg of total RNA into single-stranded cDNA in a single 20 μL reaction.

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Specifications

cDNA Synthesis Kits and Master Mixes

Shipping Condition	Dry Ice
Format	Kit
Reverse Transcriptase	MultiScribe
Reaction Format	Separate components
Final Product Type	First-Strand cDNA
Reaction Speed	120 min.
Reagent Type	Reverse Transcription
Ribonuclease H Activity	Yes
Concentration	50 U/μL
Technique	Reverse Transcription
Starting Material	RNA

Applied Biosystems™ PowerUp™ SYBR™ Green Master Mix for qPCR

Pre-formulated, optimized, universal 2X master mix for real-time PCR workflows.

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Specifications

Real Time PCR Master Mixes

Shipping Condition	Wet Ice
Polymerase	Dual-Lock Taq DNA Polymerase
Format	Tube
Product Type	Real Time PCR SYBR Master Mix
For Use With (Equipment)	7500 Fast System, 7500 System, 7900HT System, QuantStudio™ 12k Flex, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio 6 Pro, QuantStudio 7 Pro, QuantStudio™ 7 Flex, StepOne™, StepOnePlus™, ViiA™ 7 System
Sample Type	DNA
Reaction Speed	Fast or Standard
Concentration	2X
For Use With (Application)	Gene Expression
PCR Method	qPCR
Product Line	PowerUP™, SYBR™
Detection Method	SYBR

Thermo Scientific™ Phusion™ High-Fidelity DNA Polymerase (2 U/μL)

Thermo Scientific Phusion High-Fidelity DNA Polymerases set a gold standard for high performance PCR.

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Specifications

High Fidelity PCR Reagents and Kits

Shipping Condition	Dry Ice
GC-Rich PCR Performance	High
Content And Storage	• Phusion DNA Polymerase (2 U/μL) • 5X Phusion HF & GC Buffers • DMSO • 50 mM MgCl₂ solution Phusion HF Buffer and Phusion GC Buffers each provide 1.5 mM MgCl₂ in the final 1X concentration. Store at -20°C.
Fidelity (vs. Taq)	52X
Size (Final Product)	20 kb or less
Reaction Format	Standalone
Product Type	High-Fidelity DNA Polymerase
Reaction Speed	Fast
Hot Start	No
Overhang	Blunt
Concentration	2 U/μL
For Use With (Application)	Standard PCR,High-fidelity PCR

Invitrogen™ SuperScript™ IV Reverse Transcriptase

SuperScript IV Reverse Transcriptase (RT) is a proprietary MMLV mutant with superior robustness and reliability in RT reactions.

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Specifications

Reverse Transcriptases

Shipping Condition	Dry Ice
GC-Rich PCR Performance	High
Reverse Transcriptase	SuperScript IV
Final Product Type	First-Strand cDNA
Concentration	200 U/μL
Format	Stand-alone Enzyme
Size (Final Product)	Up to 12.3 kb
Reaction Format	Separate components
Reaction Speed	10 min.
Reagent Type	Reverse Transcription
Ribonuclease H Activity	Reduced
Optimal Reaction Temperature	50°C
Technique	Reverse Transcription
Starting Material	RNA

Thermo Scientific™ DreamTaq Green PCR Master Mix (2X)

Thermo Scientific DreamTaq Green PCR Master Mix (2X) is a ready-to-use solution containing DreamTaq DNA Polymerase, optimized DreamTaq Green buffer, MgCl₂, and dNTPs.

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Specifications

Standard PCR Reagents and Kits

Shipping Condition	Dry Ice
Polymerase	DreamTaq DNA Polymerase
GC-Rich PCR Performance	Low
Fidelity (vs. Taq)	1X
Reaction Format	SuperMix or Master Mix
Reaction Speed	Standard
Hot Start	No
Overhang	3'-A
Concentration	2X
For Use With (Application)	Standard PCR

Applied Biosystems™ High-Capacity RNA-to-cDNA™ Kit

The High Capacity RNA-to-cDNA Kit is a streamlined reverse transcription kit designed for optimum performance with TaqMan Gene Expression Master Mix, Power SYBR Green PCR Master Mix, and other PCR enzymes.

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Specifications

cDNA Synthesis Kits and Master Mixes

Shipping Condition	Dry Ice
Format	Kit
Reverse Transcriptase	MultiScribe
Size (Final Product)	Up to 7 kb
Reaction Format	Premixed Components
Final Product Type	First-Strand cDNA
Reaction Speed	30 to 60 min.
Reagent Type	Reverse Transcription
Ribonuclease H Activity	Yes
Optimal Reaction Temperature	37°C
Technique	Reverse Transcription
Starting Material	RNA
For Use With (Application)	Real Time PCR (qPCR),RT-PCR

TaqMan® TaqMan™ Gene Expression Assay (FAM)

Applied Biosystems TaqMan Gene Expression assays are used for quantitative real-time PCR analysis of gene expression and consist of a pair of unlabeled PCR primers and a TaqMan probe with a dye label (FAM) on the 5' end and a minor groove binder (MGB) and non-fluorescent quencher (NFQ) on the 3' end.

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Specifications

Real Time PCR Assays and Arrays

Shipping Condition	Room Temperature
GC-Rich PCR Performance	High
Fidelity (vs. Taq)	60 X
Product Type	Gene Expression Assay
Reaction Speed	Fast
Green Features	Sustainable packaging
PCR Method	qPCR
Product Line	TaqMan™
Detection Method	Primer-probe

Thermo Scientific™ RevertAid First Strand cDNA Synthesis Kit

Thermo Scientific RevertAid First Strand cDNA Synthesis Kit is a complete system for efficient synthesis of first strand cDNA from RNA templates.

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Specifications

cDNA Synthesis Kits and Master Mixes

Shipping Condition	Dry Ice
Content And Storage	• RevertAid Reverse Transcriptase • RiboLock RNase Inhibitor • 5X Reaction Buffer • dNTP Mix • Oligo(dT)₁₈ Primer • Random Hexamer Primer • Control GAPDH RNA • Forward GAPDH Primer (10 μM) • Reverse GAPDH Primer (10 μM) • Nuclease-free water Store at –20°C.
Reverse Transcriptase	RevertAid
Final Product Type	First-Strand cDNA
For Use With (Application)	RT-PCR
Format	Kit
Size (Final Product)	Up to 13 kb
Reaction Format	Separate components
Reaction Speed	60 min.
Reagent Type	Reverse Transcription
Ribonuclease H Activity	Yes
Optimal Reaction Temperature	42°C
Technique	Reverse Transcription
Starting Material	RNA

Invitrogen™ SuperScript™ II Reverse Transcriptase

Invitrogen SuperScript II Reverse Transcriptase is a genetically engineered MMLV reverse transcriptase (RT) with reduced RNase H activity and increased thermal stability compared to wild-type MMLV RT.

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Pricing and Availability
Specifications

Reverse Transcriptases

Shipping Condition	Wet Ice
GC-Rich PCR Performance	High
Reverse Transcriptase	SuperScript II
Final Product Type	First-Strand cDNA
Concentration	200 U/μL
Format	Stand-alone Enzyme
Size (Final Product)	Up to 12.3 kb
Reaction Format	Separate components
Reaction Speed	50 min.
Reagent Type	Reverse Transcription
Ribonuclease H Activity	Reduced
Optimal Reaction Temperature	42°C
Technique	Reverse Transcription
Starting Material	RNA

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Applied Biosystems™ Human GAPD (GAPDH) Endogenous Control (FAM™/MGB probe, non-primer limited) Greener Choice ProductThis product offers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”Learn More About the Greener Choice Program

Applied Biosystems™ Human GAPD (GAPDH) Endogenous Control (FAM™/MGB probe, non-primer limited)