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Applied Biosystems™ TaqMan™ Fast Universal PCR Master Mix (2X), no AmpErase™ UNG
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Requires
Template and primers
- Results in 40 minutes for 40 cycles of PCR in a 20 μL reaction volume when using the Applied Biosystems 7900HT and Fast 7500 Fast real-time PCR systems
- The optimized formulation contains AmpliTaq Fast DNA Polymerase, UP, a highly purified DNA polymerase designed to allow instant hot start, minimizing non-specific product formation and allowing room temperature reaction setup
- A proprietary ROX dye serves as a passive internal reference to normalize non-PCR-related fluorescence fluctuations, for superb precision on Applied Biosystems real-time PCR instruments
- Contains everything needed for TaqMan-based qPCR amplification and detection in a single-tube format
- TaqMan Fast Universal PCR Master Mix contains all of the components, excluding the template and primers, for superior real-time qPCR
- Compatible with TaqMan Gene Expression assays
- Store at 2°C to 8°C
Información de órdenes
Guaranteed minimum shelf life is 60 days.
Especificaciones
Especificaciones
| Concentration | 2X |
| Content And Storage | TaqMan™ Fast Universal PCR Master Mix, No AmpErase™ UNG Kit contains 2 packs of 2 tubes of 1.25 mL each sufficient for 500 20 μL PCR reactions. Store at 2-8°C Guaranteed minimum shelf life is 60 days (exact expiry date printed on product and CofA). |
| Detection Method | Primer-probe |
| Format | Tube |
| GC-Rich PCR Performance | High |
| PCR Method | qPCR |
| Polymerase | AmpliTaq DNA Polymerase |
| Reaction Speed | Fast |
| For Use With (Equipment) | 7500 Fast System, 7900HT Fast System, StepOne™, Fast Mode, StepOnePlus™, Fast Mode |
| Passive Reference Dye | ROX (Pre-mixed) |
| Mostrar más |
For Research Use Only. Not for use in diagnostic procedures.
Título del producto
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