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  7. Thermo Scientific™ Maxima Probe/ROX qPCR Master Mix (2X)

Thermo Scientific™ Maxima Probe/ROX qPCR Master Mix (2X)

Product Code. 11803923 Shop All Thermo Scientific Products
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Product Code. No. of Reactions unitSize
11803923 200 reactions 200 reactions
11813923 1,000 Reactions 1000 reactions
11823923 4,000 Reactions 4000 reactions
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Product Code. 11803923 Supplier Thermo Scientific™ Supplier No. K0231

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240.33 EUR valid until 2026-06-30
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Description

Optimize qPCR with probe chemistry using standard cycling protocols with these ready-to-use qPCR master mixes.

Maxima Probe qPCR Master Mixes are ready-to-use solutions optimized for quantitative real-time PCR. The master mixes include Maxima Hot Start Taq DNA Polymerase and dNTPs in an optimized PCR buffer. Only template and primers need to be added.

Maxima Hot Start Taq DNA Polymerase in combination with an optimized buffer ensures PCR specificity and sensitivity. dUTP is included in the mix for optional carry-over contamination control using uracil DNA glycosylase (UDG). The use of Maxima Probe qPCR Master Mixes in real-time PCR ensures reproducible, sensitive and specific quantification of genomic, plasmid, viral, and cDNA templates.

Maxima Probe qPCR Master Mixes are compatible with most real-time thermal cyclers.

The master mix includes Maxima Hot Start Taq DNA Polymerase and dNTPs in an optimized PCR buffer. It is supplemented with ROX passive reference dye. Only template and primers need to be added.Maxima Hot Start Taq DNA Polymerase in combination with an optimized buffer ensures PCR specificity and sensitivity. dUTP is included in the mix for optional carryover contamination control using uracil DNA glycosylase (UDG). ROX serves as a passive reference dye. The use of Maxima Probe/ROX qPCR Master Mix (2X) in real time PCR ensures reproducible, sensitive and specific quantification of genomic, plasmid, viral and cDNA templates. The master mix can be used with most real-time thermal cyclers, including instruments from Applied Biosystems, Eppendorf, Corbett, Bio-Rad and Roche.
  • Sensitivity—detects low copy number targets
  • Specificity—Maxima Hot Start Taq DNA Polymerase and the optimized buffer eliminate non-specific amplification and formation of primer dimers
  • Wide linear range—accurate quantification across 9 orders of magnitude
  • Universal—can be used with sequence-specific probes on most real-time thermal cyclers
  • Reproducibility and convenience—ready-to-use 2X master mix minimizes pipetting error and reduces set-up time

Recommended for:

Real-time PCR using sequence-specific probes; Real-time RT-PCR using sequence-specific probes.

TRUSTED_SUSTAINABILITY

Specifications

Concentration 2X
Content And Storage Contains:
• 2 x 1.25 mL Maxima Probe/ROX qPCR Master Mix (2X) containing Maxima Hot Start Taq DNA Polymerase and dNTPs (also dUTP) in an optimized PCR buffer with ROX passive reference dye; sufficient for 200 x 25 μL reactions.
• 2 x 1.25 mL Nuclease-Free Water

Store at -20°C.
Detection Method Primer-probe
Format Tube
GC-Rich PCR Performance High
PCR Method qPCR
Polymerase Taq DNA Polymerase
Reaction Speed Standard
For Use With (Equipment) 7000 System, 7300 System, 7700 System, 7900HT System, StepOnePlus™ System, StepOne™ System
Product Line Maxima
Product Type qPCR Master Mix
Quantity 200 reactions
Sufficient For 200 Reactions of 25 μL
For Use With (Application) Gene Expression
No. of Reactions 200 reactions
Sample Type DNA, dsDNA
Volume 2 x 1.25 mL
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For Research Use Only. Not for use in diagnostic procedures.

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