DNA Extraction and Purification
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Invitrogen™ PureLink™ HiPure Plasmid FP (Filter and Precipitator) Maxiprep Kit
The PureLink™ HiPure Plasmid FP (Filter and Precipitator) Maxiprep Kit isolates plasmid DNA, from cell pellet to ultrapure DNA, in less than one hour.
| Content And Storage | The PureLink™ HiPure Plasmid FP Maxiprep Kit includes Equilibration Buffer, Resuspension Buffer, Lysis Buffer, Precipitation Buffer, RNase A, Wash Buffer, Elution Buffer, TE Buffer, HiPure Filter Columns, and a PureLink™ HiPure Precipitator Module. The PureLink™ HiPure Precipitator Module includes HiPure Precipitators, and 5 mL and 30 mL syringes. Store all components at room temperature. |
|---|---|
| Final Product Type | Plasmid DNA |
| Isolation Technology | Anion Exchange Resin |
| Column Type | Filter Column |
| System Type | PureLink™ |
| Purity | Transfection Grade |
| High-throughput Compatibility | Not High-throughput Compatible (Manual) |
| For Use With (Application) | Next-Generation Sequencing, Transfection, Cloning, Sequencing, Transformation, Nucleic Acid Labeling, PCR, In Vitro Transcription |
| Test Time | 1 hr. |
| Prep Scale | > 200 μg (Large-Scale) Plasmid DNA |
| Plasmid | <40kb, Low Copy Plasmid, High Copy Plasmid, BAC |
| Target | Plasmid DNA |
| Format | Column |
| Product Type | Plasmid FP MaxiPrep Kit |
| Sample Type | Bacterial Culture |
| Yield | 1000 μg |
| Elution Volume | 15 mL |
| Product Line | PureLink |
Invitrogen™ PureLink™ Fast Low-Endotoxin Maxi Plasmid Purification Kit
The PureLink Fast Low-Endotoxin Maxi Plasmid Purification Kit uses next-generation columns with advanced silica membranes that enable isolation of transfection grade (<1 EU/μg) plasmid DNA in about 30 minutes.
| Shipping Condition | Room Temperature |
|---|---|
| Content And Storage | • Resuspension buffer, store at 4°C upon reciept • Lysis buffer, store at room temperature • Precipitation buffer, store at room temperature • Binding buffer, store at room temperature • Wash buffer 1, store at room temperature • Wash buffer 2 (concentrate), store at room temperature • Elution buffer, store at room temperature • Column assemblies, store at room temperature • Syringe filters, store at room temperature • Syringe plungers, store at room temperature • Collection tubes, store at room temperature |
| Format | Kit |
| Product Type | Plasmid Purification Kit |
| Isolation Technology | Silica Column |
| Scale | Maxi |
| For Use With (Application) | Cloning, PCR |
| Test Time | 30 min. |
| Product Line | PureLink |
| Prep Scale | Maxi input culture of 100-500 mL to obtain a yield of up to 850 μg |
Macherey-Nagel™ NucleoBond™ Finalizer Large for desalting and concentration of plasmid DNA
Proven syringe filter for speeding up anion exchange plasmid preparations.
Macherey-Nagel™ NucleoBond™ Finalizer Large Plus for desalting and concentration of plasmid DNA
Proven syringe filter for speeding up anion exchange plasmid preparations.
Invitrogen™ PureLink™ Fast Low-Endotoxin Midi Plasmid Purification Kit
The PureLink Fast Low-Endotoxin Midi Plasmid Purification Kit uses next-generation columns with advanced silica membranes that enable isolation of transfection grade (<1 EU/μg) plasmid DNA in about 30 minutes.
| Shipping Condition | Room Temperature |
|---|---|
| Content And Storage | • Resuspension buffer, store at 4°C upon reciept • Lysis buffer, store at room temperature • Precipitation buffer, store at room temperature • Binding buffer, store at room temperature • Wash buffer 1, store at room temperature • Wash buffer 2 (concentrate), store at room temperature • Elution buffer, store at room temperature • Column assemblies, store at room temperature • Syringe filters, store at room temperature • Syringe plungers, store at room temperature • Collection tubes, store at room temperature |
| Format | Kit |
| Product Type | Plasmid Purification Kit |
| Isolation Technology | Silica Column |
| Scale | Midi |
| For Use With (Application) | Cloning, PCR |
| Test Time | 30 min. |
| Product Line | PureLink |
| Prep Scale | Midi scale input culture of 25-100 mL to obtain yields of up to 200 μg |
Invitrogen™ PureLink™ HiPure Precipitator Module
The PureLink™ HiPure Precipitator allows fast, simple, and efficient desalting and concentration of plasmid DNA isolated using anion-exchange chromatography.
| Shipping Condition | Room Temperature |
|---|---|
| Target | Plasmid DNA |
| Content And Storage | The PureLink™ HiPure Precipitator Module includes HiPure Precipitators, and 5 mL and 30 mL syringes. Store all components at room temperature. |
| Format | Syringe |
| Product Type | Precipitator Module |
| Final Product Type | Plasmid DNA |
| Sample Type | Bacterial Culture |
| Isolation Technology | Anion Exchange Resin |
| High-throughput Compatibility | Not High-throughput Compatible (Manual) |
| For Use With (Application) | Next-Generation Sequencing, Transfection, Cloning, Sequencing, Transformation, Nucleic Acid Labeling, PCR, In Vitro Transcription |
| Test Time | 5 min. |
| Product Line | PureLink |
| Prep Scale | > 200 μg (Large-Scale) Plasmid DNA |